Purified anti-human CD25 Antibody

Pricing & Availability
Clone
BC96 (See other available formats)
Workshop
V T-072
Other Names
Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BC96_Pure_071805
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with purified CD25 (clone BC96) (solid line) or purified mouse IgG1, κ isotype control (dotted line).
  • BC96_Pure_071805
    PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with purified CD25 (clone BC96) (solid line) or purified mouse IgG1, κ isotype control (dotted line).
Cat # Size Price Quantity Avail. Save
302602 100 µg 40 CHF
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Baboon, Chimpanzee, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations
  1. Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
  2. Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed
  3. Volchenkov R, et al. 2017. Front Immunol. 0.818055556. PubMed
  4. Sharabi A, et al. 2019. JCI Insight. 4:e126294. PubMed
RRID
AB_314272 (BioLegend Cat. No. 302602)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 05.01.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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