- TUJ1 (See other available formats)
- Regulatory Status
- Other Names
- CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
|801221||25 tests||121 CHF|
|801222||100 tests||319 CHF|
Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Host Species
- This antibody was raised against microtubules derived from rat brain.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7, Western blotting8, and spatial biology (IBEX)9,10.
This antibody is well characterized and highly reactive to neuron specific Class III ß-tubulin (ßIII). TUJ1 does not identify ß-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8.
(PubMed link indicates BioLegend citation)
- Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC)
- Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed
- Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed
- Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed
- Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC)
- Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed
- Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC)
- Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
AB_2892489 (BioLegend Cat. No. 801221)
AB_2892489 (BioLegend Cat. No. 801222)
- Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD.
Tissue distribution: central and peripheral nervous system.
Cellular distribution: cytosol, cytoskeleton and nucleus.
- Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance.
- Alpha tubulin, kinesin and dynein.
- Cell Type
- Mature Neurons, Neural Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Antigen References
1. Zhao X, et al. 2017. Med Sci Monit. 22: 3915.
2. Lebok P, et al. 2016. Oncol Lett. 11(3):1987.
3. Du J, et al. 2015. BMC Cancer. 15:536. PubMed
4. Rogue DM., et al. 2013. Clin Exp Metastasis. 31(1): 101.
5. Ploussard G, et al. 2010. Cancer Res. 70(22):9253. PubMed
- Gene ID
- 10381 View all products for this Gene ID
- View information about Tubulin beta-3 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
|Alexa Fluor® 488 anti-Tubulin β 3 (TUBB3)||TUJ1||ICC, IHC-P, ICFC, 3D IHC, SB|
|Purified anti-Tubulin β 3 (TUBB3)||TUJ1||IHC-P, WB, ICC, FC|
|Alexa Fluor® 594 anti-Tubulin β 3 (TUBB3)||TUJ1||ICC, 3D IHC|
|Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3)||TUJ1||ICC, ICFC, 3D IHC|
|HRP anti-Tubulin β 3 (TUBB3)||TUJ1||WB, IHC-P|
|Biotin anti-Tubulin β 3 (TUBB3)||TUJ1||WB, IHC-P|
|APC anti-Tubulin β 3 (TUBB3)||TUJ1||ICFC|
|PE/Cyanine7 anti-Tubulin β 3 (TUBB3)||TUJ1||ICFC|
|PerCP/Cyanine5.5 anti-Tubulin β 3 (TUBB3)||TUJ1||ICFC|
|PE anti-Tubulin β 3 (TUBB3)||TUJ1||ICFC|
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Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Alexa Fluor® 488 anti-Tubulin β 3 (TUBB3)
Purified anti-Tubulin β 3 (TUBB3)
Alexa Fluor® 594 anti-Tubulin β 3 (TUBB3)
Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3)
HRP anti-Tubulin β 3 (TUBB3)
Biotin anti-Tubulin β 3 (TUBB3)
APC anti-Tubulin β 3 (TUBB3)
PE/Cyanine7 anti-Tubulin β 3 (TUBB3)
PerCP/Cyanine5.5 anti-Tubulin β 3 (TUBB3)
PE anti-Tubulin β 3 (TUBB3)