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PE anti-human CD34 Antibody

Pricing & Availability
Clone
581 (See other available formats)
Regulatory Status
RUO
Workshop
V MA27
Other Names
Gp105-120, My10
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_581_PE_072909.jpg
Human peripheral blood mononuclear cells stained with 581 PE (lower panel) or PE mouse IgG1 isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
  • A_581_PE_072909.jpg
    Human peripheral blood mononuclear cells stained with 581 PE (lower panel) or PE mouse IgG1 isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
  • B_581_PE_072909.jpg
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Cat # Size Price Quantity Check Availability Save
343505 25 tests 123 CHF
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343506 100 tests 273 CHF
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press.
  2. Felschow DM, et al. 2001. Blood 97:3768.
  3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
  6. Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Product Citations
  1. Werner-Klein M, et al. 2014. PLoS One. 9:97860. PubMed
  2. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  3. Gao G, et al. 2022. Am J Cancer Res. 12:1934. PubMed
  4. Zhang B, et al. 2022. Stem Cells Int. 2022:2236250. PubMed
  5. Han S, et al. 2017. Leukemia. 10.1038/leu.2017.72. PubMed
  6. Liu W, et al. 2018. Theranostics. 8:5575. PubMed
  7. Zhang J, et al. 2022. Int J Mol Sci. 23:. PubMed
  8. Albayrak E, et al. 2021. J Cell Physiol. 236:8122. PubMed
  9. Wu L, et al. 2018. Oncol Lett. 15:9507. PubMed
  10. Ogunlade O, et al. 2020. J Biomed Opt. 25:1. PubMed
  11. Bunis DG, et al. 2021. Cell Reports. 34(1):108573. PubMed
  12. Stowe CL, et al. 2019. eLife. 0.333333333333333. PubMed
  13. Shastri A, et al. 2018. J Clin Invest. 128:5479. PubMed
  14. Vyas P, et al. 2019. Sci Rep. 9:5549. PubMed
  15. Damkham N, et al. 2022. Stem Cell Res Ther. 13:467. PubMed
  16. Keshtkar S, et al. 2021. Stem Cells Int. 8857457:2020. PubMed
  17. Turan RD, et al. 2020. Sci Rep. 10:7994. PubMed
  18. Xu L, et al. 2022. Int J Mol Sci. 23:. PubMed
  19. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  20. Wang W, et al. 2022. Int J Mol Sci. 23:. PubMed
  21. Fei D, et al. 2021. Front Cell Dev Biol. 9:687258. PubMed
  22. Sugimura R, et al. 2020. Biomed Microdevices. 34:22. PubMed
  23. Pan C, et al. 2019. Int J Mol Med. 44:1629. PubMed
  24. Zheng C, et al. 2021. J Extracell Vesicles. 10:e12109. PubMed
  25. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  26. Chijimatsu R, et al. 2022. Regen Ther. 21:52. PubMed
RRID
AB_1731937 (BioLegend Cat. No. 343505)
AB_1731862 (BioLegend Cat. No. 343506)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selectin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.

Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

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Go To Top Version: 2    Revision Date: 03.18.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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