Brilliant Violet 421™ anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
1_2B8_BV421_1_040411
  • 1_2B8_BV421_1_040411
  • 2_2B8_BV421_2_040411
  • 3_50_Mouse_Gut_EpCAM_CD117_B220
    Confocal image of C57BL/6 mouse small intestine sample acquired using the IBEX method of highly multiplexed antibody-based imaging: EpCAM (cyan) in Cycle 1, B220 (purple) in Cycle 2, and CD117 (yellow) in Cycle 2. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
  • 4_69_Human_LN_CD117_CD66b_CD35
    Confocal image of human lymph node sample acquired using the IBEX method of highly multiplexed antibody-based imaging: CD35 (blue) in Cycle 6, CD117 (yellow) in Cycle 6, and CD66b (magenta) in Cycle 11. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Compare all formats See Brilliant Violet 421™ spectral data
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105827 125 µL 165 CHF
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105828 500 µL 385 CHF
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

SB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  2. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  3. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  4. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  5. Liu L, et al. 2022. Cancers (Basel). 14:. PubMed
  6. Thiele K, et al. 2015. Am J Pathol. Available online 5 August 2015. PubMed
  7. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  8. Wu W, et al. 2014. Proc Natl Acad Sci U S A. 111:4221. PubMed
  9. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  10. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  11. Tyrkalska SD, et al. 2019. Immunity. 51:50. PubMed
  12. Carpenter RS, et al. 2020. Nat Commun. 3.029166667. PubMed
  13. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  14. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  15. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  16. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  17. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  18. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  19. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  20. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  21. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  22. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  23. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  24. Gao X, et al. 2021. Nature. 589:591. PubMed
  25. Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
  26. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  27. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  28. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  29. Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
  30. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  31. Strattan E, et al. 2020. Frontiers in Immunology. 2.131944444. PubMed
  32. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  33. Abdel Malik R, et al. 2017. Circ Res. 120:99. PubMed
RRID
AB_10898120 (BioLegend Cat. No. 105827)
AB_11204256 (BioLegend Cat. No. 105828)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Other Formats

View All CD117 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD117 (c-Kit) 2B8 FC
Biotin anti-mouse CD117 (c-Kit) 2B8 FC
FITC anti-mouse CD117 (c-Kit) 2B8 FC
PE anti-mouse CD117 (c-Kit) 2B8 FC
PE/Cyanine5 anti-mouse CD117 (c-Kit) 2B8 FC
Purified anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
PE/Cyanine7 anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 488 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Alexa Fluor® 647 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Pacific Blue™ anti-mouse CD117 (c-Kit) 2B8 FC
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit) 2B8 FC
PerCP anti-mouse CD117 (c-kit) 2B8 FC
APC/Cyanine7 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 421™ anti-mouse CD117 (c-Kit) 2B8 FC,SB
Purified anti-mouse CD117 (c-Kit) (Maxpar® Ready) 2B8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 711™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 594 anti-mouse CD117 (c-Kit) 2B8 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 510™ anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 785™ anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-A0012 anti-mouse CD117 (c-kit) 2B8 PG
Brilliant Violet 605™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 700 anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-B0012 anti-mouse CD117 (c-Kit) 2B8 PG
TotalSeq™-C0012 anti-mouse CD117 (c-Kit) 2B8 PG
Spark NIR™ 685 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 650™ anti-mouse CD117 (c-kit) 2B8 FC
Spark Red™ 718 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Go To Top Version: 3    Revision Date: 04.19.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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