Alexa Fluor® 488 anti-mouse IgG2b Antibody

Pricing & Availability
Clone
RMG2b-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2b
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
RMG2b-1_A488_IgG2b_Antibody_1_062620
Human peripheral blood lymphocytes were stained with CD4 (clone OKT4) (filled histogram) or purified mouse IgG2b (clone MPC-11) isotype control (open histogram) followed by anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 488.
  • RMG2b-1_A488_IgG2b_Antibody_1_062620
    Human peripheral blood lymphocytes were stained with CD4 (clone OKT4) (filled histogram) or purified mouse IgG2b (clone MPC-11) isotype control (open histogram) followed by anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 488.
  • RMG2b-1_A488_IgG2b_Antibody_2_062620
    C57BL/6 mouse frozen brain section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature, permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of anti-GFAP (clone 2E1.E9) purified overnight at 4°C, followed by 2.5 µg/mL of anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 488 (green) for 1 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with the MetaMorph® software.
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
406717 25 µg 70,00 CHF
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406718 100 µg 150,00 CHF
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Description

The RMG2b-1 monoclonal antibody reacts with immunoglobulin G2b (IgG2b) in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. The RMG2b-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
RRID
AB_2876732 (BioLegend Cat. No. 406717)
AB_2876732 (BioLegend Cat. No. 406718)

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Go To Top Version: 1    Revision Date: 06.26.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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