Alexa Fluor® 488 anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
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Product Citations
publications
30-F11_Alx488_090507
C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 488
  • 30-F11_Alx488_090507
    C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 488
See Alexa Fluor® 488 spectral data
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103121 25 µg 85,00 CHF
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103122 100 µg 195,00 CHF
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
  1. Gutknecht M, et al. 2017. Nat Commun. 10.1038/s41467-017-00488-6. PubMed
  2. Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed
  3. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  4. Peng W, et al. 2018. Mol Cell Biol. 38:e00427. PubMed
  5. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  6. Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed
  7. Wu X, et al. 2018. Cell. 172:423. PubMed
  8. Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
  9. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  10. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  11. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  12. Kazakevych J, et al. 2019. Sci Rep. 9:10410. PubMed
  13. Wang Y, et al. 2019. Front Cell Infect Microbiol. 9:286. PubMed
  14. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  15. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  16. Kazakevych J, et al. 2020. Genome Biol. 21:64. PubMed
  17. Sakai M, et al. 2020. Immunity. 51(4):655-670. PubMed
  18. Giorgetti E, et al. 2020. Cell Reports. 29(6):1539-1554.e7.. PubMed
  19. Pang M, et al. 2009. J Immunol. 182:7001. PubMed
  20. Ferrari G, et al. 2011. Invest Ophthalmol Vis Sci. 52:2532. PubMed
  21. Ye Y, et al. 2011. Am J Physiol Lung Cell Mol Physiol. 300:L216. PubMed
  22. Braun D, et al. 2013. J Biol Chem. 288:2689. PubMed
  23. Khandelwal P, et al. 2013. PLoS One. 8:64193. PubMed
  24. Marko C, et al. 2014. Invest Ophthalmol Vis Sci . 55:291. PubMed
  25. RL M, et al. 2015. Proc Natl Acad Sci U S A. 112:6506-6514. PubMed
  26. Kuo P, et al. 2016. J Am Heart Assoc. 5: 002610. PubMed
  27. Ding H, et al. 2016. Nat Commun. 7:11533. PubMed
  28. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  29. Chen I, et al. 2016. Sci Rep. 6:27195. PubMed
  30. Tian F, et al. 2016. Nat Commun. 7:13283. PubMed
  31. Mizbani A, et al. 2016. Development. 143: 4137 - 4148. PubMed
  32. Duarte J, et al. 2016. J Immunol. 197(12):4838-4847. PubMed
  33. Bornes L, et al. 2021. Life Sci Alliance. 4:00. PubMed
  34. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
  35. Markworth JF, et al. 2020. JCI Insight. 5:00. PubMed
  36. Masschelein E, et al. 2020. Skelet Muscle. 10:21. PubMed
  37. Burns JC, et al. 2020. eLife. 9:00. PubMed
  38. DeDreu J, et al. 2020. FASEB J. 34:9316. PubMed
  39. Dobson HE, et al. 2020. Mucosal Immunol. 0.901388889. PubMed
  40. Reed-Geaghan EG, et al. 2020. J Exp Med. 217:00:00. PubMed
  41. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  42. Yip HYK, et al. 2020. Molecular Cell. 80(2):279-295.e8. PubMed
  43. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  44. Yip HYK, et al. 2021. STAR Protocols. 2(3):100765. PubMed
  45. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  46. Dos Santos Dias L, et al. 2021. PLoS Pathog. e1009324:17. PubMed
  47. Preda MB, et al. 2021. Cell Death Dis. 12:566. PubMed
  48. Jung Y, et al. 2021. Nat Commun. 12:3872. PubMed
RRID
AB_493532 (BioLegend Cat. No. 103121)
AB_493531 (BioLegend Cat. No. 103122)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 11.03.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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