Alexa Fluor® 488 anti-His Tag Antibody

Pricing & Availability
Clone
J099B12 (See other available formats)
Regulatory Status
RUO
Other Names
Histidine Tag
Isotype
Mouse IgG1, κ
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Product Citations
publications
J099B12_A488_HisTag_Antibody_100617
Hela cells transfected with multi-tag plasmid were fixed with cold methanol for 15 minutes at -20°C and then blocked with 5% FBS for 60 minutes. Cells were then stained with either (A) 1:500 (1 µg/ml) Alexa Fluor® 488 mouse IgG1, κ isotype control antibody (Cat no. 400133), or (B, C) 1:500 (1 µg/ml), (D) 1:1000 (0.5 µg/ml) or (E) 1:2500 (0.2 µg/ml) Alexa Fluor® 488 anti-His antibody (green), and incubated overnight at 4°C. Nuclei were counterstained with DAPI (blue, Cat no. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (S): (A) 1/15, (B, D, E) 1/50, (C) 1/150.
  • J099B12_A488_HisTag_Antibody_100617
    Hela cells transfected with multi-tag plasmid were fixed with cold methanol for 15 minutes at -20°C and then blocked with 5% FBS for 60 minutes. Cells were then stained with either (A) 1:500 (1 µg/ml) Alexa Fluor® 488 mouse IgG1, κ isotype control antibody (Cat no. 400133), or (B, C) 1:500 (1 µg/ml), (D) 1:1000 (0.5 µg/ml) or (E) 1:2500 (0.2 µg/ml) Alexa Fluor® 488 anti-His antibody (green), and incubated overnight at 4°C. Nuclei were counterstained with DAPI (blue, Cat no. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (S): (A) 1/15, (B, D, E) 1/50, (C) 1/150.
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability Save
652509 25 µg 135,00 CHF
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Description

This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
All species (6x His tag epitope)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.2 - 0.5 μg/ml (Dilution 1:1000-1:2500) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
RRID
AB_2716151 (BioLegend Cat. No. 652509)

Antigen Details

Function
Recombinant poly-histidine tag detection
Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about His Tag on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10.05.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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