Purified anti-His Tag Antibody

Pricing & Availability
Clone
J099B12 (See other available formats)
Regulatory Status
RUO
Other Names
Histidine Tag
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
aJ099B12_WB_042612
Western blot analysis of cell lysates from HeLa cells transfected without or with His-tagged plasmid using His Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
  • aJ099B12_WB_042612
    Western blot analysis of cell lysates from HeLa cells transfected without or with His-tagged plasmid using His Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
  • bJ099B12_WB_042612
    Immunofluorescence of HeLa cells transfected without (A) or with (B) His-tagged protein plasmid using anti-His Mouse primary Antibody. Alexa Fluor® 594 (Red) Goat anti-Mouse IgG (Cat. No. 405326) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective. Exposure time (Seconds) for (A) is 1/30, and (B) is 1/100.
  • cJ099B12_IF_042612
    Insect cell supernatant expressing His-tagged mouse CCL22 was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-His Tag antibody (clone J099B12). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
652501 25 µg 75 CHF
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652502 100 µg 150 CHF
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652505 1 mg 575 CHF
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Description

This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
All species (6x His tag epitope)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml (Dilution 1:1000 - 1:5000). For immunocytochemistry, a concentration range of 0.1 - 0.5 μg/ml (Dilution 1:1000 - 1:5000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Product Citations
  1. Shigemori S, et al. 2017. J Dairy Sci. 10.3168/jds.2017-12872. PubMed
  2. Mamedov T, et al. 2017. PLoS One.. 10.1371/journal.pone.0183589. PubMed
  3. Namai F, et al. 2017. Curr Microbiol. 10.1007/s00284-017-1354-3. PubMed
  4. Mamedov T, et al. 2019. PLoS One. 14:e0213438. PubMed
  5. Liu L, et al. 2019. J Cell Biol. 218:644. PubMed
  6. Jain RW, et al. 2018. Cell Rep. 25:3342. PubMed
  7. Williford JM, et al. 2019. Sci Adv. 5:eaay1357. PubMed
  8. Keshwani M, et al. 2015. Biochem J. 472: 329 - 338. PubMed
  9. Yumoto K, et al. 2020. Antioxidants (Basel). 9:00. PubMed
RRID
AB_11204080 (BioLegend Cat. No. 652501)
AB_11204427 (BioLegend Cat. No. 652502)
AB_2564271 (BioLegend Cat. No. 652505)

Antigen Details

Function
Recombinant poly-histidine tag detection
Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about His Tag on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03.18.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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