Flow Cytometry Controls

Veri-Cells™...

Are most appropriate when you need reference/control cells for longitudinal, multi-center studies.

Products

Reference laboratories, clinical research organizations, and multi-center clinical trials, among other institutions, need reliable controls to monitor assay performance and variability for longitudinal studies. Veri-Cells™ is our line of lyophilized control cell products, which can be used to help monitor data quality and aid in reproducibility in multi-center and longitudinal studies. 

Veri-Cells™ feature:

• Exceptional long term stability
• Scatter profile similar to fresh samples
• Validation for the detection of over 150 markers

For more helpful information on Veri-Cells™, visit the FAQ page.

Sections:

Veri-Cells™ Heavy Metal (Ta) PBMC

Veri-Cells™ Activated (Cytokine) PBMC

Veri-Cells™ Activated (Surface)

Veri-Cells™ Phospho PBMC (MAPK/ERK Pathway)

Veri-Cells™ CD34 PBMC

Veri-Cells™ Leukocytes

Veri-Cells™ CD4-Low PBMC

Veri-Cells™ PBMC

Veri-Cells™ Heavy Metal (Ta) PBMC

Veri-Cells™ Heavy Metal (Ta) PBMCs have been labeled with the heavy metal tantalum. When mixed with sample cells, they can be separated from the cells of interest using the tantalum signal. They have been verified to stain for commonly tested surface markers including CD3, CD4, CD8, CD14, CD16, CD19, CD38, CD123, and HLA-DR.

Veri-Cells™ Heavy Metal (Ta) PBMCs were mixed with whole blood from different donors. Mixed samples were stained with a heavy-metal labeled anti-CD14 antibody and run on a CyTOF®. Median intensity of CD14 signal was measured for the blood sample alone (top row) and normalized to the median intensity of CD14 signal on Veri-Cells™ in each sample (bottom row). Normalized median values are indicated. Data provided by Adeeb Rahman, PhD, Icahn School of Medicine at Mt. Sinai.

Veri-Cells™ Activated (Cytokine) PBMC

Veri-Cells™ Activated (Cytokine) PBMC have been activated with our Cell Activation Cocktail (with Brefeldin A), which includes phorbol 12-myristate 13-acetate (PMA) and Ionomycin, for 6 hours. They have been validated to stain for activation-induced cytokines including IL-2, IL-3, IL-4, IL-5, IL-8, IL-13, IL-17, GM-CSF, IFN-γ, and TNF-α, along with surface markers typically expressed on human PBMCs. Cells do not require additional fixation nor permeabilization prior to cytokine staining, thus saving more time in your experiment.

Veri-Cells™ Activated (Cytokine) PBMCs were stained with anti-CD3 FITC and either anti-TNF-α PerCP/Cyanine5.5, anti-IL-17A BV421™, anti-GM-CSF Pacific Blue™, or anti-IFN-γ APC. Plots were gated based on staining with the corresponding isotype controls as indicated.

Veri-Cells™ Activated (Surface)

Veri-Cells™ Activated (Surface) have been activated with Concanavalin A for 72 hours. This makes them ideal for the detection of T cell activation markers, including CD25, CD69, CD134, CD279, CD366, and TIGIT.

Veri-Cells™ Activated (Surface) PBMCs were stained with anti-CD4 FITC and either anti-CD25 PerCP/Cy5.5, anti-CD134 APC, anti-CD69 APC, or anti-TIGIT APC. Plots were generated on CD3⁺ cells and gated based on staining with the corresponding isotype control as indicated.

Veri-Cells™ Phospho PBMC (MAPK/ERK Pathway)

Veri-Cells™ Phospho PBMC are stimulated with PMA/Ionomycin for 15 minutes. This product includes both stimulated and vehicle control cells for the staining of RAS mitogen-activated kinase pathways, including phospho-S6, phospho-p38, and phospho-ERK1/2.

Veri-Cells™ Phospho PBMC (MAPK/ERK Pathway) vehicle control (open histogram) or stimulated cells (closed histogram) were stained with anti-phospho S6-Alexa Fluor® 488 (left), anti-phospho p38-PE (center) or anti-human phospho ERK-Alexa Fluor® 647 (right). The histograms are gated on the lymphocyte population.

Veri-Cells™ CD34 PBMC

Veri-Cells™ CD34 PBMC contains mixture of CD34+ KG-1a cells and human PBMCs to help verify CD34 antibody binding without the need for artificial enrichment or the collection of a large number of events.

Veri-Cells™ CD34 PBMCs were stained with anti-CD45 (clone HI30) FITC and anti-CD34 (clone 561) PE. Dot plots have excluded doublets from analysis.

Veri-Cells™ Leukocytes 

Unlike Veri-Cells™ PBMC and CD4-Low PBMC, Veri-Cells™ Leukocytes contain neutrophils and eosinophil populations. These cells can be used as controls or reference material to monitor expression of granulocyte markers such as CD15 and CD16.

Veri-Cells™ Leukocytes were surface stained with anti-CD8 Alexa Fluor® 700, then fixed and permeabilized before staining with anti-Granzyme B FITC, and anti-Perforin PE. Scatter profile of the cells is shown on the left.

Veri-Cells™ CD4-Low PBMC

Veri-Cells™ CD4-Low PBMC are specially formulated to contain a lower frequency of CD4+ cells, similar to that observed in patients with CD4 immunodeficiency. The expected frequency of CD3+ CD4+ cells can be found on the certificate of analysis for that lot.

Veri-Cells™ PBMCs and CD4-low PBMCs were stained with anti-CD4 PE/Cy7 and anti-CD3 FITC and the frequency of CD4⁺ cells is indicated. Plots were first gated on the lymphocyte population.

Veri-Cells™ PBMC

Veri-Cells™ PBMC consist of lyophilized human PBMCs validated for over 150 surface markers and intracellular targets and exhibit a scatter profile similar to freshly isolated cells. Please refer to the Validation Tab to view the full listing of markers and clones that can be used with Veri-Cells™ PBMC.