BioLegend Epitope Tag Products
When incorporated into the protein of interest, epitope tags make protein detection and identification very robust and easy. One of the earliest epitope tags was poly-arginine. The sequence of five arginine residues allowed purification on a cation exchange resin. Now, the number of epitope tags has increased considerably. BioLegend epitope tag antibodies, peptide tags, and affinity gels are extremely specific, highly characterized and generate clear, quantitative results.These tags can be used for many applications such as:
  • Western Blot
  • Immunoprecipitation
  • Protein Purification
  • Flow Cytometry
  • Immunofluorescence Microscopy
Featured Products and Data   Common Epitope Tags   FAQs   Background
Affinity Gels and Matrices
Epitope tag antibodies can be conjugated to beads (agarose, sepharose, metal, etc.) in order facilitate isolation or immunoprecipitation of tagged proteins. BioLegend offers several affinity gels and matrices, including c-Myc, 6-His, DYKDDDDK, GST, GFP, and HA. These matrices can be re-used for multiple rounds of purification and display excellent specificity at an economical price.

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DYKDDDDK and c-Myc Affinity Gel Data
SDS-PAGE gel image of protein purified from the 1st or 10th rounds by either BioLegend anti-DYKDDDDK agarose or a competitor’s equivalent product. Final lane contains ladder.SDS-PAGE gel image of protein purified from the 1st to 10th rounds by BioLegend anti-c-Myc agarose. First and last lanes contain ladder. There is no loss of binding capacity even after ten rounds of re-use.

Recombinant Peptide Tags and Posi-Tag
BioLegend's Posi-Tag Epitope Tag Protein can be utilized as a positive control in Western blotting experiments where many epitope tags are commonly used. Posi-Tag represents proteins fused in the following order: GST, T7, HSV, c-Myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag, HA, and 6-His, and has an approximate molecular weight of 45 kDa.

We also offer recombinant peptide tags that can be used during competition experiments to ensure specificity of your antibody. Alternatively, these synthetic peptides can be used to help elute the corresponding tagged protein from its affinity matrix.

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Western blot showing several BioLegend epitope tag antibodies and their detection of 1 μg of Posi-Tag Epitope Tag Protein. All monoclonal antibodies were used at a 1:1000 dilution. Poly9248 was used at a dilution of 1:50000.

Epitope Tag Antibodies
The epitope tag antibodies we provide are extremely specific and provide outstanding performance for protein purification, Western blotting, and immunoprecipitation applications.

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Purified anti-HA.11 Epitope Tag AntibodyPurified anti-DYKDDDDK Tag AntibodyPurified anti-His Tag Antibody
Western blot of Clone 16B12. Lane 1: Molecular weight marker; Lane 2: Posi-tag control Protein (Cat # 931301); Lane 3: HA.11 overexpressing cell lysate; Lane 4: Untransfected cell lysate.Cell extracts from untransfected 293T cells (lane 1) or 293T cells transfected with a plasmid encoding DYKDDDDK-tagged protein (lane 2), using anti-DYKDDDDK, clone L5.Insect cell supernatant expressing His-tagged mouse CCL22 was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-His Tag antibody (clone J099B12). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.
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