Ultra-LEAF™ Purified anti-human Neurturin Antibody

Pricing & Availability
Clone
A16100E (See other available formats)
Regulatory Status
RUO
Other Names
NRTN, NTN
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16100E_ULEAF_Neurturin_Antibody_042120.png
Recombinant human GFRα2 (black circles) binds to immobilized recombinant human Neurturin (Cat. No. 760304). Anti-human Neurturin antibody (purple squares, clone A16100E) inhibits the binding in a dose-dependent manner while the isotype control (grey triangles, Cat. No. 402202) does not have the effect. This antibody blocks the binding of 0.5 µg/mL recombinant human GFRα2 protein to 0.5 µg/mL of immobilized recombinant human Neurturin with an ND50 = 0.2 - 5 µg/mL.
  • A16100E_ULEAF_Neurturin_Antibody_042120.png
    Recombinant human GFRα2 (black circles) binds to immobilized recombinant human Neurturin (Cat. No. 760304). Anti-human Neurturin antibody (purple squares, clone A16100E) inhibits the binding in a dose-dependent manner while the isotype control (grey triangles, Cat. No. 402202) does not have the effect. This antibody blocks the binding of 0.5 µg/mL recombinant human GFRα2 protein to 0.5 µg/mL of immobilized recombinant human Neurturin with an ND50 = 0.2 - 5 µg/mL.
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935003 100 µg $245.00
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935004 1 mg $650.00
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Description

Neurturin belongs to the GDNF family of ligands, which consists of 4 members: GDNF, Neurturin, Artemin, and Persephin. GDNF family members orchestrate multiple aspects of the development and maintenance of the central and peripheral nervous systems. These GDNF family ligands signal through a multicomponent receptor system composed of a common signaling component RET (receptor tyrosine kinase) and one of the high affinity binding coreceptors GFRα (α1-α4). Neurturin is a GDNF family member that functions as a ligand for the GFRα2 and RET complex. Neurturin is a potent neurotrophic factor that can promote the survival of numerous neuronal populations, which include the sympathetic, nodose, and dorsal root ganglion sensory neurons. Neurturin is synthesized as a precursor protein that is cleaved to release the carboxy-terminal domain. The carboxy-terminal domain contains seven conserved cysteine residues that are necessary for the formation of the cysteine-knot and the single interchain disulfide bond. The functional form of human Neurturin is a disulfide-linked homodimer of the carboxy-terminal 102 amino acid residues. Studies have shown positive results for Neurturin in protecting and repairing dopaminergic neurons in various models of Parkinson's disease. In association with mutations of the RET gene, genetic variations in Neurturin may contribute to Hirschsprung's disease.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Neurturin
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding between recombinant human GFRα2 and recombinant human Neurturin (Cat. No. 760304). The ND50 range is 0.2 - 5 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
RRID
AB_2861117 (BioLegend Cat. No. 935003)
AB_2861118 (BioLegend Cat. No. 935004)

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

Neurturin is a secreted protein that is expressed in both neuronal tissues and non-neuronal tissues such as the pancreas, heart, and hair follicles.

Function
It is a neurotrophic factor and promotes the survival of sympathetic, nordose, and dorsal root ganglion sensory neurons.
Interaction
Neurturin targets multiple central and peripheral neurons including the midbrain dopaminergic, sympathetic, and sensory neurons.
Ligand/Receptor
Interacts with GDNF family receptor α-2 (GFRα2). Has a lower affinity with GFRα1.
Cell Type
Embryonic Stem Cells, Mature Neurons, Neural Stem Cells, Neurons
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors
Antigen References
  1. Kotzbauer PT, et al. 1996. Nature 6608:467-70.
  2. Doray B, et al. 1998. Hum. Mol. Genet. 7:1449-52.
  3. Milbrandt J, et al. 1998. Neuron 20:245-53.
  4. Baloh RH, et al. 1998. Neuron. 21:1291-302.
  5. Sariola H, Saarma M. 2003. 116:3855-3862.
  6. Ruiz-Ferrer M, et al. 2011. J. Mol. Med. (Berl) 5:471-80.
  7. Lindholm D, et al. 2016. Cell Mol. Life Sci. 7:1365-79.
Gene ID
4902 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Neurturin
Specificity Alt (DOES NOT SHOW ON TDS):
Neurturin
UniProt
View information about Neurturin on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 04/21/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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