- S+16a (See other available formats)
- Regulatory Status
- Other Names
- ART2.2, Art2b, Art, Rt6, Rt-6, Ly92b, Rt6-2
- Llama VH Ig domain
- Ave. Rating
- Submit a Review
- Product Citations
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ARTC2, also known as ART2.2, is a 35 kD, GPI-anchored, and cell surface ADP-ribosyltransferase that is highly expressed by regulatory T cells (Tregs) and natural killer T (NKT) cells. ARTC2 is enzymatically active at 4°C. Through the ribosylation of the ion channel P2X7, ARTC2 mediates NAD+-induced cell death (NICD) and the proteolytic shedding of CD27 and CD62L. Blocking the activity ARTC2 with Treg-Protector™ improves the recovery of Tregs and NKT cells during their isolation from lymphatic and peripheral tissues.Product Details
- Antibody Type
- Expression vector encoding full length mouse ARTC2.
- 1 mg/mL S+16a Nanobody in PBS. Purity ≥ 95%, as determined by Coomassie stained SDS-PAGE. Less than 0.01 ng of endotoxin/µg of protein, as determined by the LAL method.
- Treg-Protector ™ is sterilized by filtration through a 0.22 µm membrane and contains no preservatives.
- 1.0 mg/ml
- Storage & Handling
- Store between 2°C and 8°C. Do not freeze. Keep sterile.
Block - Quality tested
- Recommended Usage
We recommend diluting Treg-Protector™ 1:4 with sterile PBS and administer 200 µL (50 µg) intravenously (i.v.) of diluted Treg-Protector™ per mouse. Euthanize the mouse and begin the cell preparation 15 - 60 minutes after the injection. Intraperitoneal (IP) injection is also effective at similar doses (validated by developer).
- Application Notes
Treg-Protector™ (S+16a Nanobody) is a recombinant llama VH Ig domain that binds to the ADP-ribosyltransferase ARTC2, which reversibly blocks its enzymatic activity and protects ARTC2+ cells from NAD+-induced cell death (NICD), which is caused by the release of NAD+ by cells that die during the preparation of primary lymphocytes. The S+16a nanobody has been demonstrated to protect a variety of cell types including Tregs, NKT cells, and tissue-resident memory T cells8.
(PubMed link indicates BioLegend citation)
- Rissiek B, et al. 2014. J. Leuko. Biol. 95:543.
- Bannas P, et al. 2014. Contrast Media Mol. Imaging 9:135.
- Lischke T, et al. 2013. Eur. J. Immunol. 43:1828.
- Hubert S, et al. 2010. J. Exp. Med. 207:2561.
- Scheuplein F, et al. 2010. J. Autoimmun. 34:145.
- Scheuplein F, et al. 2009. J. Immunol. 182:2898.
- Koch-Nolte F, et al. 2007. FASEB J. 21:3490.
- Fernandez-Ruiz D, et al. 2016. Immunity. 45:889.
- Product Citations
AB_2565485 (BioLegend Cat. No. 149801)
AB_2819899 (BioLegend Cat. No. 149803)
AB_2565494 (BioLegend Cat. No. 149802)
- GPI-anchored surface protein, 35 kD
Subsets of mature T cells, regulatory T cells (Tregs), natural killer T cells (NKTs), and γδ T cells.
- Cell Type
- NKT cells, T cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Antigen References
1. Menzel S, et al. 2013. FEBS J. 280:3543.
2. Bannas P, et al. 2011. Mol. Immunol. 48:1762.
3. Chen YG, et al. 2011. J. Immunol. 186:4278.
4. Adriouch S, et al. 2007. J. Immunol. 179:186.
5. Bannas P, et al. 2005. Blood 105:3663.
- Gene ID
- 11872 View all products for this Gene ID
- View information about ARTC2 on UniProt.org
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