Purified anti-Myosin Heavy Chain Antibody

Pricing & Availability
Clone
H11 (See other available formats)
Regulatory Status
RUO
Other Names
MHC
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
H11_PURE_Myosin_Heavy_Chain_Antibody_1_090921.png
Total cell lysates (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 μg/mL (1:500 dilution) of purified anti-Myosin Heavy Chain antibody (clone H11) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664803) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • H11_PURE_Myosin_Heavy_Chain_Antibody_1_090921.png
    Total cell lysates (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 μg/mL (1:500 dilution) of purified anti-Myosin Heavy Chain antibody (clone H11) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664803) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • H11_PURE_Myosin_Heavy_Chain_Antibody_1_090921.png
    ICC staining of purified mouse IgM, κ isotype ctrl antibody (Cat. No. 401602) (panel A) and purified anti-Myosin Heavy Chain antibody (clone H11) (panel B) on HeLa cells. The cells were fixed with 4% PFA and permeabilized with 100% methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
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922703 25 µg $100.00
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922704 100 µg $250.00
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Description

Myosin heavy chain is an actin-based motor protein with an approximate molecular weight of 223 kD. Myosin heavy chain can be split into 1 light meromysin and 1 heavy meromysin chain (and further into 2 globular subfragments and 1 rod-shaped subfragment). This protein is highly expressed in muscle. Motor proteins that convert chemical energy derived from ATP hydrolysis into mechanical force that drives motile processes such as cytokinesis, vesicular transport, and cellular locomotion. Myosin heavy chain is modified by acetylation in cysteine residues in the S1 domain for myosin ATPase activity. Muscle myosin is a hexameric protein consisting of 2 heavy chain subunits and 2 alkali light chain subunits and 2 regulatory light chain subunits that forms filaments.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated against a complex mixture of phosphoproteins extracted from Madin-Darby Canine Kidney Cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When using this clone for ICC, we recommend fixing cells with 4% PFA and permeabilizing with methanol. The clone was not compatible with mysosin heavy chain staining when cells were fixed/permeabilized with methanol only or 4% PFA + Triton X-100.

RRID
AB_2894519 (BioLegend Cat. No. 922703)
AB_2894519 (BioLegend Cat. No. 922704)

Antigen Details

Structure
Actin-based motor protein, approximately 223 kD. Myosin heavy chain can be split into 1 light meromysin and 1 heavy meromysin chain (and further into 2 globular subfragments and 1 rod-shaped subfragment).
Distribution

Highly expressed in muscle.

Function
Motor proteins that convert chemical energy derived from ATP hydrolysis into mechanical force that drives motile processes such as cytokinesis, vesicular transport, and cellular locomotion/ Cysteine residues in the S1 domain are alkylated for myosin ATPase activity
Interaction
Muscle myosin is a hexameric protein consisting of 2 heavy chain subunits and 2 alkali light chain subunits and 2 regulatory light chain subunits. Forms filaments.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Weiss A, et al. 1999. Proc. Natl. Acad. Sci. USA 96:2598.
  2. Schiaffino S, et al. 1996. Physiol. Rev. 76:371
Gene ID
NA
UniProt
View information about Myosin Heavy Chain on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/22/2021

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