Anti-Myosin Heavy Chain Antibody (Previously Covance catalog# MMS-460R)

Pricing & Availability
Clone
H11 (See other available formats)
Other Names
MHC
Previously
Covance Catalog# MMS-460R
Isotype
Mouse IgM
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Product Citations
publications
1_H11_Myosin_Heavy_Chain-_Antibody_WB_1_123118_updated.png
Total cell lysates (15 µg total protein) from A431, HaCat, HeLa, U937, and NTERA-2 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with a 1:2500 dilution of Purified anti-Myosin Heavy Chain Antibody, clone H11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 1_H11_Myosin_Heavy_Chain-_Antibody_WB_1_123118_updated.png
    Total cell lysates (15 µg total protein) from A431, HaCat, HeLa, U937, and NTERA-2 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with a 1:2500 dilution of Purified anti-Myosin Heavy Chain Antibody, clone H11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 2_H11_Anti-Myosin_Ascites_ICC_112817
    Immunofluorescence of HUVEC cells with (A) mouse isotype control (negative) or (B-D) myosin heavy chain antibody (clone H11). Alexa Fluor® 488 (green) Goat anti-mouse IgG (Cat. No. 405319) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (seconds) for (A) is 1/10, and (B-D) is 1/15. Concentrations for (A, B) is 1/100, (C) is 1/250 and (D) is 1/500.
Cat # Size Price Quantity Avail. Save
922701 100 µL $218
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Description

Myosin heavy chain is an actin-based motor protein with an approximate molecular weight of 223 kD. Myosin heavy chain can be split into 1 light meromysin and 1 heavy meromysin chain (and further into 2 globular subfragments and 1 rod-shaped subfragment). This protein is highly expressed in muscle. Motor proteins that convert chemical energy derived from ATP hydrolysis into mechanical force that drives motile processes such as cytokinesis, vesicular transport, and cellular locomotion. Myosin heavy chain is modified by acetylation in cysteine residues in the S1 domain for myosin ATPase activity. Muscle myosin is a hexameric protein consisting of 2 heavy chain subunits and 2 alkali light chain subunits and 2 regulatory light chain subunits that forms filaments.

Product Details
Technical data sheet

Product Details

Reactivity
Mammalian
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated against a complex mixture of phosphoproteins extracted from Madin-Darby Canine Kidney Cells.
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is a dilution of 1:1000 - 1:2500. For immunocytochemistry, a dilution of 1:1000 - 1:250 is recommended. For immunoprecipitation, the suggested use of this reagent is a dilution of 1:150. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunofluorescence (ICC) and immunoprecipitation (IP).

*Predicted MW = 205 kD
**Recommend using extracts prepared in buffers containing 0.5% Triton X-100 and 0.5% sodium deoxycholate.

This antibody binds specifically to denatured as well as native myosin heavy chain extracts and will not react with amphibian myosins. 

Application References

(PubMed link indicates BioLegend citation)
  1. Chew TL, et al. 2002. J. Cell Biol. 156:543. (ICC) PubMed
  2. Warren SL, et al. 1988. Mol Cell Biol. 8:632.
Product Citations
  1. Chew T, et al. 2002. J Cell Biol. 156:543-553. PubMed
  2. Aaron JS, et al. 2018. J Cell Sci. 131. PubMed
  3. Zehrer A, et al. 2018. J Immunol. 201:1748. PubMed
RRID
AB_2565417 (BioLegend Cat. No. 922701)

Antigen Details

Structure
Actin-based motor protein, approximately 223 kD. Myosin heavy chain can be split into 1 light meromysin and 1 heavy meromysin chain (and further into 2 globular subfragments and 1 rod-shaped subfragment).
Distribution

Highly expressed in muscle.

Function
Motor proteins that convert chemical energy derived from ATP hydrolysis into mechanical force that drives motile processes such as cytokinesis, vesicular transport, and cellular locomotion.
Interaction
Muscle myosin is a hexameric protein consisting of 2 heavy chain subunits and 2 alkali light chain subunits and 2 regulatory light chain subunits. Forms filaments.
Modification
Alkylation.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Weiss A, et al. 1999. P. Natl. Acad. Sci. USA 96:2598.
2. Schiaffino S, et al. 1996. Physiol. Rev. 76:371 (review)

Regulation
Cysteine residues in the S1 domain are alkylated for myosin ATPase activity.
Gene ID
NA
UniProt
View information about Myosin Heavy Chain on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 12/31/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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