Purified anti-mouse Ly49H Antibody

Pricing & Availability
Clone
3D10 (See other available formats)
Regulatory Status
RUO
Other Names
Klra8, Cmv1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3D10_Purified_Ly49H _Antibody_FC_1_091912
C57BL/6 mouse splenocytes were stained with CD49b (DX5) Pacific Blue™ and purified Ly49H (clone 3D10, top) or mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 FITC.
  • 3D10_Purified_Ly49H _Antibody_FC_1_091912
    C57BL/6 mouse splenocytes were stained with CD49b (DX5) Pacific Blue™ and purified Ly49H (clone 3D10, top) or mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 FITC.
  • 3D10_Purified_Ly49H _Antibody_FC_2_091912
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144702 200 µg $195.00
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Description

Ly49H, also known as Cmv1 and Klra8, is a 28.3 kD protein.  It is expressed on NK cells (but not on NKT cells) from C57BL/6 mice (but not Balb/c or DBA/2 mice).  Expression of Ly49H has been associated with resistance to MCMV infection in C57BL/6 mice.  Modulation of Ly49H expression by its antibody made MCMV-resistant C57BL/6 mice susceptible to infection.  Unlike Ly49A, Ly49H lacks the ITIM (immunoreceptor tyrosine-based inhibitory motif) but has ITAM (immunoreceptor tyrosine-based activation motif), which is associated with DAP-12 (KARAP, killer cell activating motif).

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C1498-Ly49A/H transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunoprecipation1, and in vitro2 and in vivo3 blocking activity.  The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 144717 & 144718).

Application References

(PubMed link indicates BioLegend citation)
  1. Guseva NV, et al. 2010. Eur. J. Immunol. 40:2618. (IP)
  2. Orr MT, et al. 2009. J. Exp. Med. 206:807. (Block)
  3. Rodriguez M, et al. 2004. J. Immunol. 173:6312. (Block)
Product Citations
  1. Niogret C, et al. 2019. Nat Commun. 10:1444. PubMed
  2. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  3. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  4. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
RRID
AB_2561549 (BioLegend Cat. No. 144702)

Antigen Details

Structure
28.3 kD
Distribution

NK cells but not NKT cells

Function
Interacts with m157, a cytomegalovirus-encoded protein
Ligand/Receptor
m157
Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Gosselin P, et al. 1999. J. Leuko. Bio. 66:165.
2. Lee SH, et al. 2001. Nat. Genet. 28:42.
3. Daniels KA, et al. 2001. J. Exp. Med. 194:29.

Gene ID
16639 View all products for this Gene ID
UniProt
View information about Ly49H on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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