Purified anti-mouse CD107b (Mac-3) Antibody

Pricing & Availability
Clone
M3/84 (See other available formats)
Regulatory Status
RUO
Other Names
CD107b, LAMP-2
Isotype
Rat IgG1, κ
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Product Citations
publications
M3/84
Thioglycolate-elicited peritoneal macrophages stained with M3/84 PE
  • M3/84
    Thioglycolate-elicited peritoneal macrophages stained with M3/84 PE
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108501 50 µg $70.00
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108502 500 µg $220.00
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Description

Mac-3 is a 110 kD type I membrane glycoprotein, also known as CD107b and LAMP-2. It is expressed on lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. In the bone marrow, few cells display Mac-3 antigen on the surface, but a large proportion express Mac-3 in the cytoplasm. CD107b has been identified as a ligand for galaptin, an S-type lectin present in the extracellular matrix. Mac-3/CD107b is upregulated in some tumors and increased expression has been correlated with enhanced metastatic potential.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, ICC, IHC-P, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-4 and immunohistochemical staining of acetone-fixed frozen sections5,6 and paraformaldehyde-fixed paraffin-embedded sections9-11.

Application References

(PubMed link indicates BioLegend citation)
  1. Springer TA. 1981. J. Biol. Chem. 256:3833. (IP)
  2. Ho MK, et al. 1983. J. Biol. Chem. 258:636. (IP)
  3. Chen JW, et al. 1985. J. Cell Biol. 101:85. (IP)
  4. Ralph P, et al. 1983. J. Immunol. 130:108. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Kano M, et al. 1998. Transplantation 65:837. (IHC)
  7. Terrazas LI,et al.2005. Int J Parasitol. 35:1349. PubMed
  8. Hayashida A, et al. 2011. J. Biol Chem. 286:3288. PubMed
  9. Vollmar P, et al. 2010. J. Immunol. 185:6338. (IHC)
  10. Odorisio T, et al. 2002. J. Cell Sci. 115:2559. (IHC)
  11. Nessler S, et al. 2007. Brain 130:2186. (IHC)
Product Citations
  1. Orimo T, et al. 2019. Int Immunol. 31:657. PubMed
  2. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  3. Sonner JK, et al. 2019. Nat Commun. 3.803472222. PubMed
  4. Terrazas LI, et al. 2005. Int J Parasit. 35:1349. PubMed
  5. Hayashida A, et al. 2011. J Biol Chem. 286:3288. PubMed
  6. Luo Y, et al. 2017. Cell Res. 10.1038/cr.2017.8. PubMed
  7. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  8. Blriot C, et al. 2021. Immunity. :. PubMed
  9. Sagawa T, et al. 2021. Environ Res. 110722:195. PubMed
RRID
AB_313382 (BioLegend Cat. No. 108501)
AB_313383 (BioLegend Cat. No. 108502)

Antigen Details

Structure
110 kD
Distribution

Macrophages, some myeloid cell lines

Function
Differentiation and activation
Cell Type
Macrophages
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Springer TA. 1981. J. Biol. Chem. 256:3833.
2. Ho MK, et al. 1983. J. Biol. Chem. 258:636.
3. Ralph P, et al. 1983. J. Immunol. 130:108.

Gene ID
16784 View all products for this Gene ID
UniProt
View information about CD107b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10/22/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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