Purified anti-IL-27 p28 Antibody

Pricing & Availability
Clone
B0315A10 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-27 Subunit Alpha, IL-27 (28K subunit)
Isotype
Mouse IgG2b
Ave. Rating
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Product Citations
publications
B0315A10_Purified_IL-27_p28_Antibody_WB_050115
Western blot analysis of 50 ng recombinant human IL-27 (lane 1) and recombinant mouse IL-27 (lane 2) using anti-IL-27 P28 antibody (clone B0315A10) and HRP anti-mouse IgG antibody.
  • B0315A10_Purified_IL-27_p28_Antibody_WB_050115
    Western blot analysis of 50 ng recombinant human IL-27 (lane 1) and recombinant mouse IL-27 (lane 2) using anti-IL-27 P28 antibody (clone B0315A10) and HRP anti-mouse IgG antibody.
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676902 100 µg $195.00
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Description

IL-27 is a heterodimeric cytokine that is composed of two subunits (p28 and EBI3). It plays a critical role in T-cell proliferation and differentiation. It also drives rapid clonal expansion of naive but not memory CD4 T cells. IL-27 is a potent inhibitor of Th17 cell development and IL-17 production. IL-27 subunit p28 alone is able to inhibit the production of IL-17 by CD4 and CD8 T cells. Furthermore, IL-27 plays an important role in antitumor and antiangiogenic activity through the increase in the production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-27.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565723 (BioLegend Cat. No. 676902)

Antigen Details

Structure
A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40 related protein) and p28 (a newly discovered IL-12 p35-related protein).
Distribution
Monocytes, macrophages, and dendritic cells (DCs).
Function
T cell proliferation and differentiation.
Interaction
Activated T cells, resting natural killer cells (NK), resting NKT cells, neutrophils, eosinophils, mast cells, hepatic stellate cells, hepatoma cells, and hepatocytes.
Ligand/Receptor
IL-27RA, also known as WSX-1 or TCCR, paired with gp130.
Cell Type
Monocytes, Macrophages, Dendritic cells, B cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Pflanz S, et al. 2002. Immunity 16:779.
2. Owaki T, et al. 2005. J. Immunol. 175:2191.
3. Batten M, et al. 2006. Nat. Immunol. 7:929.
4. Villarino AV, et al. 2006. J. Immunol. 176:237.
5. Stumhofer JS, et al. 2007. Nat. Immunol. 8:1363.
6. Awasthi A, et al. 2007. Nat. Immunol. 8:1380.
7. Pot C, et al. 2009. J. Immunol. 183:797.
8. Bender H, et al. 2009. Hepatology 50:585.
9. Bosmann M and Ward PA. 2013. J. Leukoc. Biol. 94:1159.

Gene ID
246778 View all products for this Gene ID
UniProt
View information about IL-27 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/01/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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