Purified anti-human IL-23 (p19) Antibody

Pricing & Availability
Clone
HLT2736 (See other available formats)
Regulatory Status
RUO
Other Names
p19p40, JKA3 induced upon T cell activation
Isotype
Mouse IgG1, κ
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Product Citations
publications
HLT-2736_080505
Linker-free, recombinant human IL-23 was resolved by electrophoresis, transferred to nitrocellulose and probed with the anti-IL23 p19 specific antibody HLT2736 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. The HLT2736 recognizes the p19-specific subunit of human IL-23. This antibody will also recognize human IL-23 (p19 +p40 subunits) in recombinant IL-23 containing a linker moiety.
  • HLT-2736_080505
    Linker-free, recombinant human IL-23 was resolved by electrophoresis, transferred to nitrocellulose and probed with the anti-IL23 p19 specific antibody HLT2736 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. The HLT2736 recognizes the p19-specific subunit of human IL-23. This antibody will also recognize human IL-23 (p19 +p40 subunits) in recombinant IL-23 containing a linker moiety.
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511201 50 µg $100.00
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511202 500 µg $260.00
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Description

The HLT2736 clone recognizes human IL-23, a heterodimeric cytokine composed of the p40 subunit shared with IL-12 and a specific p19 subunit. This antibody recognizes heterodimeric IL-23, but does not react with the p40 subunit alone or recombinant IL-12(p70) when used as a capture antibody in ELISA. IL-23 is secreted by activated dendritic cells and macrophages and has been shown to enhance IFN-γ secretion by memory (CD45RO) T cells in an IL-2 dependent manner. IL-23 may also induce unique Th subset (designated ThIL-17) that secretes the cytokines IL-17, IL-6, TNF and low levels of IFN-γ. IL-23 has been shown to promote immunity to mycobacteria and has been shown to be upregulated in response to some viral infections and in psoriatic lesions. IL-23 binds to the IL-23 receptor comprised of a β1 subunit shared with the IL-12 receptor and IL-23-specific subunit. This antibody has been shown to be useful for Western blotting and immunohistochemistry using formalin-fixed, paraffin embedded tissues with (catalyzed signal amplification).

Product Details
Technical data sheet

Product Details

Reactivity
Human, IL-23 p19.
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human recombinant p40 and p19 with elastin linker
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting applications, a concentration range of 0.5 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified HLT2736 antibody has been shown to be useful for Western blotting and immunohistochemistry using formalin-fixed, paraffin embedded tissues (with catalyzed signal amplification). This antibody weakly stains frozen sections. This clone does not cross-react with mouse.

Application References

(PubMed link indicates BioLegend citation)
  1. Doherty SD, et al. 2006. Arch. Dermatol. Res. 298(7):353-6.
  2. Ohyama H, et al. 2009. J. Dent. Res. 88:633. (IHC-P) PubMed
  3. Katsifis GE, et al. 2009. Am. J. Pathol. 175:1167. (IHC-P) PubMed
  4. Schlapbach C, et al. 2011. J. Am. Acad. Dermatol. 65:790. PubMed
Product Citations
  1. Li H, et al. 2018. Nat Commun. 9:2830. PubMed
  2. Katsifis G, et al. 2009. Am J Pathol. 175:1167. PubMed
  3. Ohyama H, et al. 2009. J Dent Res. 88:633. PubMed
  4. Tripodo C, et al. 2010. Am J Pathol. 177:792. PubMed
  5. Schlapbach C, et al. 2011. J Am Acad Dermatol. 65:790. PubMed
  6. Bohnacker S, et al. 2020. Mol Immunol. 120:126. PubMed
RRID
AB_416323 (BioLegend Cat. No. 511201)
AB_416324 (BioLegend Cat. No. 511202)

Antigen Details

Structure
Member of the IL-12 cytokine family, heterodimeric cytokine composed of p40 subunit shared with IL-12 and a specific p19 subunit
Distribution

Secreted by activated dendritic cells and macrophages

Function
Enhances IFN-γ secretion by memory (CD45RO) T cells in an IL-2 dependent manner; may induce unique Th subset (designated ThIL-17) secreting IL-17, IL-6, TNF and low levels of IFN-γ in EAE model; shown to promote immunity to mycobac
Ligand/Receptor
IL-23 receptor (β1 subunit of IL-12 receptor and IL-23-specific subunit)
Cell Type
Dendritic cells, Macrophages
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Parham C, et al. 2002. J. Immunol. 168:5699.
2. Verreck FA, et al. 2004. Proc. Natl. Acad. Sci. USA 101:4560.
3. Lee E, et al. 2004. J. Exp. Med. 199:125.
4. Oppmann B, et al. 2000. Immunity 13:715.

Regulation
Upregulated in viral infections such as HSV, increased in psoriatic lesions in monocytes and mature dendritic cells
Gene ID
51561 View all products for this Gene ID
UniProt
View information about IL-23 p19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/09/2018

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