Purified anti-c-Myc Antibody (Previously Covance catalog# MMS-164P)

Pricing & Availability
Clone
9E11 (See other available formats)
Regulatory Status
RUO
Other Names
Myc proto-oncogene protein, proto-oncogene c-Myc, transcription factor p64, class E basic helix-loop-helix protein 39, avian myelocytomatosis viral oncogene homolog, v-myc, myc-related translation/localization
Previously
Covance Catalog# MMS-164P
Isotype
Mouse IgG2a
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Product Citations
publications
9E11_PURE_c-Myc_Antibody_1_071921
Whole cell extracts (15 µg total protein) from HeLa cells mixed with the indicated amount of recombinant Posi-tag epitope tag protein were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-c-Myc antibody (clone 9E11) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 9E11_PURE_c-Myc_Antibody_1_071921
    Whole cell extracts (15 µg total protein) from HeLa cells mixed with the indicated amount of recombinant Posi-tag epitope tag protein were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-c-Myc antibody (clone 9E11) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 9E11_PURE_c-Myc_Antibody_2_071921
    ICC staining of purified anti-c-Myc antibody (clone 9E11) on HeLa cells transfected with multi-tag plasmid containing c-Myc epitope sequence. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100, and blocked with 5% fetal bovine serum in PBS for 1 hour. The cells were then incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 488 goat anti-mouse IgG (green) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20X objective. Scale bar: 100 µm
  • 9E11_PURE_c-Myc_Antibody_3_071921
    IHC staining of anti-c-Myc antibody (clone 9E11) on formalin-fixed paraffin-embedded human bladder tissue. Following antigen retrieval using 0.1M Tris-buffered saline w/ Tween 20 (Cat. No. 925501), the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slide was mounted with ProLong&reade; Gold Antifade Mountant. The image was captured with a 20x objective. Scale bar: 100 µm
  • 9E11_PURE_c-Myc_Antibody_4_071921
    Whole cell extracts (250 µg total protein) prepared from HeLa cells mock-transfected (-) or transfected (+) with multi-tag plasmid containing c-Myc epitope sequence were immunoprecipitated overnight with 2.5 µg of purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400101) or purified anti-c-Myc antibody (clone 9E11). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with purified anti-c-Myc antibody (clone 9E11) at 1.0 µg/mL (1:1000 dilution). Lane M: Molecular weight marker.
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904401 100 µL $303.00
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Description

c-Myc is a naturally occurring protein that is often used as a protein modification and tag in order to simplify the labeling and detection of proteins. Antibodies against c-Myc recognize both naturally occurring c-Myc and overexpressed proteins containing the c-Myc epitope tag fused to targeted proteins allowing for specific detection in western blotting, immunoprecipitation, and immunostaining techniques.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC, IHC-P, IP - Verified
Purification - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 9E11 monoclonal antibody recognizes the sequence 408-420 AEEQKLISEEDL of human c-myc.

This clone recognizes a non-specific band in BL21 E. coli lysates

BioLegend offers two clones against c-Myc, 9E10 and 9E11. When both clones were tested in parallel for WB, ICC, and IP, each clone performed similarly, with 9E11 showing slightly better performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Halme A, et al. 2004. Cell. 116:405.
  2. Kolodziej P, Young R. 1991. Meth. Enzymol. 194:508.
  3. Locker AP, et al. 1989. Brit. J. Cancer. 60:669.
Product Citations
  1. Wu Y, et al. 2016. Nat Struct Mol Biol. 10.1038/nsmb.3284. PubMed
RRID
AB_2565039 (BioLegend Cat. No. 904401)

Antigen Details

Cell Type
Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Stem Cells, Transcription Factors
Gene ID
4609 View all products for this Gene ID
UniProt
View information about c-Myc on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 07/19/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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