Purified anti-c-Myc Antibody (Previously Covance catalog# MMS-164P)

Pricing & Availability
Clone
9E11 (See other available formats)
Other Names
Myc proto-oncogene protein, proto-oncogene c-Myc, transcription factor p64, class E basic helix-loop-helix protein 39, avian myelocytomatosis viral oncogene homolog, v-myc, myc-related translation/localization
Previously
Covance Catalog# MMS-164P
Isotype
Mouse IgG2a
Ave. Rating
Submit a Review
Product Citations
publications
9E11_Purified_c-myc_052219.png
0.1 µL of Recombinant Posi-Tag Epitope Tag Protein (Cat No. 931301) was resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:4,000 dilution) of Purified anti-c-Myc Antibody, clone 9E11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution Lane M: Molecular Weight marker.
  • 9E11_Purified_c-myc_052219.png
    0.1 µL of Recombinant Posi-Tag Epitope Tag Protein (Cat No. 931301) was resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:4,000 dilution) of Purified anti-c-Myc Antibody, clone 9E11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
904401 100 µL $303
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

c-Myc is a naturally occurring protein that is often used as a protein modification and tag in order to simplify the labeling and detection of proteins. Antibodies against c-Myc recognize both naturally occurring c-Myc and overexpressed proteins containing the c-Myc epitope tag fused to targeted proteins allowing for specific detection in western blotting, immunoprecipitation, and immunostaining techniques.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, ICC, IHC, Purification

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:1,000
IP: 1:150

Application Notes

This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC) and immunohistochemistry (IHC).

The 9E11 monoclonal antibody recognizes the sequence 408-420 AEEQKLISEEDL of human c-myc.

This clone recognizes a non-specific band in BL21 E. coli lysates

Application References

(PubMed link indicates BioLegend citation)
  1. Halme A, et al. 2004. Cell. 116:405.
  2. Kolodziej P, Young R. 1991. Meth. Enzymol. 194:508.
  3. Locker AP, et al. 1989. Brit. J. Cancer. 60:669.
Product Citations
  1. Wu Y, et al. 2016. Nat Struct Mol Biol. 10.1038/nsmb.3284. PubMed
RRID
AB_2565039 (BioLegend Cat. No. 904401)

Antigen Details

Cell Type
Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Stem Cells, Transcription Factors
Gene ID
4609 View all products for this Gene ID
UniProt
View information about c-Myc on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 05/22/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Forgot your password? Reset Password
Request an Account