PE/Cyanine7 anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
H57-597_PECy7_122607
C57BL/6 mouse splenocytes stained with H57-597 PE/Cyanine7
  • H57-597_PECy7_122607
    C57BL/6 mouse splenocytes stained with H57-597 PE/Cyanine7
See PE/Cyanine7 spectral data
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109221 25 µg $85.00
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109222 100 µg $200.00
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Krementsov DN, et al. 2018. Front Immunol. 9:1622. PubMed
  2. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  3. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  4. Liang Z, et al. 2017. Autophagy. 14:505. PubMed
  5. Kyburz A, et al. 2017. Clin Exp Allergy. 47:1331. PubMed
  6. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  7. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  8. Udden SN et al. 2019. eLife. 8 pii: e40396. PubMed
  9. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  10. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  11. Man SM et al. 2016. Cell. 167(2):382-396 . PubMed
  12. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
  13. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  14. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  15. Martens R, et al. 2020. Nat Commun. 11:1114. PubMed
  16. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  17. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  18. Sencio V, et al. 2020. Cell Reports. 30(9):2934-2947. PubMed
  19. Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed
  20. Somerville TD, et al. 2020. eLife. 9:e53381.. PubMed
  21. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  22. Nieuwenhuijze A, et al. 2014. Am J Pathol. 184:184. PubMed
  23. Sell S, et al. 2015. PLoS Pathog. 11:1004481. PubMed
  24. Ribeiro-Gomes F, et al. 2015. Cell Death Dis. 6: 2018. PubMed
  25. Sandrock I, et al. 2015. PLoS One. 10: 0145010. PubMed
  26. Thornton T, et al. 2016. Nat Commun. 7:10553. PubMed
  27. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  28. Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed
  29. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  30. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  31. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  32. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  33. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  34. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  35. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  36. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  37. Baars MJD, et al. 2020. Eur J Immunol. . PubMed
RRID
AB_893627 (BioLegend Cat. No. 109221)
AB_893625 (BioLegend Cat. No. 109222)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/06/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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