PE anti-mouse CD284 (TLR4) Antibody

Pricing & Availability
Clone
SA15-21 (See other available formats)
Regulatory Status
RUO
Other Names
Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2
Isotype
Rat IgG2a, κ
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Product Citations
publications
SA15-21_PE_TLR4_Antibody_FC_021913
Thioglycollate-elicited C57BL/6 mouse peritoneal macrophages were stained with TLR4 (clone SA15-21) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • SA15-21_PE_TLR4_Antibody_FC_021913
    Thioglycollate-elicited C57BL/6 mouse peritoneal macrophages were stained with TLR4 (clone SA15-21) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
See PE spectral data
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145403 25 µg $100.00
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145404 100 µg $215.00
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Description

Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells, and T cells. TLR4 has been recognized as critical for host recognition of LPS in conjunction with MD2.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS-stimulated TLR4/MD-2/CD14 cotransfected NKR cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.1 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.1 Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation1. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.

Additional reported applications (for the relevant formats) include: immunoprecipitation1 and inhibition of LPS-induced hepatocyte apoptosis2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).

Application References

(PubMed link indicates BioLegend citation)
  1. Akashi S, et al. 2003. J. Exp. Med. 198:1035. (IP)
  2. Wakabayashi Y, et al. 2006. J. Immunol. 177:1772. (Block)
  3. Visintin A, et al. 2006. J. Leukoc. Biol. 80:1584. (FC)
  4. Shibata T, et al. 2011. Int. Immunol. 23:503. (FC)
Product Citations
  1. Arya SB, et al. 2018. J Biol Chem. 293:9892. PubMed
  2. Xu X, et al. 2018. Cell. 175:1336. PubMed
  3. Gotoh K et al. 2018. Cell reports. 25(7):1800-1815 . PubMed
  4. Schappe MS, et al. 2018. Immunity. 48:59. PubMed
  5. Zanoni I et al. 2017. Immunity. 47(4):697-709 . PubMed
  6. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
  7. Lin SY, et al. 2017. Autophagy. 14:778. PubMed
  8. Haratani K, et al. 2019. J Clin Invest. 130:374. PubMed
  9. Li Y, et al. 2019. J Biol Chem. 294:2744. PubMed
  10. Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed
  11. Kim K, et al. 2015. Infect Immun . 83:1150. PubMed
  12. Yang L, et al. 2015. PLoS One. 10:126576. PubMed
  13. Wei L, et al. 2015. J Biol Chem. 290: 16633 - 16652. PubMed
  14. Noya V, et al. 2017. Sci Rep. 7:40615. PubMed
  15. Hu J, et al. 2020. Theranostics. 7.154166667. PubMed
  16. Cilenti F, et al. 2021. Immunity. . PubMed
RRID
AB_2561873 (BioLegend Cat. No. 145403)
AB_2561874 (BioLegend Cat. No. 145404)

Antigen Details

Structure
Type I transmembrane protein complex
Distribution
Macrophages, B cells and T cells
Interaction
Activates the innate immune system against Gram-negative pathogens by recognition of LPS
Ligand/Receptor
Bacterial LPS
Cell Type
Macrophages, B cells, T cells, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Brennan TV, et al. 2012. Blood 120:2899.
2. Wantia N, et al. 2011. PLoS One 6:e26101.
3. Loser K, et al. 2010. Nat. Med. 16:713.
4. Kang S, et al. 2009. Blood 113:2605.

Gene ID
21898 View all products for this Gene ID
UniProt
View information about CD284 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 02/07/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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