FITC anti-human CD41/CD61 Antibody

Pricing & Availability
Clone
PAC-1 (See other available formats)
Regulatory Status
RUO
Other Names
gpIIb/IIIa complex, integrin αIIbβ3
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
PAC-1_FITC_glycoproteinIIb-IIIa_Antibody_FC_1_062014.jpg
ADP activated human enriched platelets (top) or resting platelets (bottom) were co-stained with CD62P PE and anti-human glycoprotein IIb/IIIa complex (clone PAC-1) FITC.
  • PAC-1_FITC_glycoproteinIIb-IIIa_Antibody_FC_1_062014.jpg
    ADP activated human enriched platelets (top) or resting platelets (bottom) were co-stained with CD62P PE and anti-human glycoprotein IIb/IIIa complex (clone PAC-1) FITC.
  • PAC-1_FITC_glycoproteinIIb-IIIa_Antibody_FC_2_062014.jpg
See FITC spectral data
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362803 25 tests $125.00
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362804 100 tests $275.00
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Description

The gpIIb/IIIa complex, also known as integrin αIIbβ3, is expressed by megakaryocytes and platelets. It undergoes conformational changes upon activation by substances, such as ADP, thrombin, and TXA2, which subsequently reveals a ligand binding site that is specific for fibrinogen, von Willebrand factor (vWF), fibronectin, and vitronectin. This conformational change is crucial for platelet-platelet adhesion. Ligand bound gpIIb/IIIa complex promotes platelet aggregation. This complex plays an important role in hemostasis and is a target for developing therapeutic antibodies in immune thrombocytopenia.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunoprecipitation1,2, Western blotting2 and in vitro blocking of platelet aggregation2.

 

The PAC-1 antibody reacts with activation-induced conformational epitope of the integrin aIIbß3. PAC-1 binding to fibrinogen is pH and Ca2+ dependent, and will not bind to platelets that have been fixed.  We recommend staining in buffers free of Ethylenediaminetetraacetic acid (EDTA) at a pH of 6.5 or higher.  Sodium citrate may be used as an anticoagulant for blood collection3.

Application References

(PubMed link indicates BioLegend citation)
  1. Shattil SJ, et al. 1987. Blood. 70:307. (IP)
  2. Shattil SJ, et al. 1985. J. Biol. Chem. 260:11107. (Block, IP, WB)
  3. Shattil SJ, et al. 1986. Blood. 68: 1224-31.
Product Citations
  1. Lien L, et al. 2017. Int J Mol Sci. 10.3390/ijms18071500. PubMed
  2. Qi Y, et al. 2018. J Hematol Oncol. 11:117. PubMed
  3. Costa B, et al. 2019. Blood Adv. 3:1092. PubMed
  4. Bhat A, et al. 2020. Hepatol Commun. 4:50. PubMed
  5. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  6. Lu WJ, et al. 2019. Sci Rep. 9:18852. PubMed
RRID
AB_2563899 (BioLegend Cat. No. 362803)
AB_2563900 (BioLegend Cat. No. 362804)

Antigen Details

Structure
α/β transmembrane heterodimer of the ubiquitous integrin superfamily
Distribution

Resting platelets; activated platelets express a conformationally modified complex that can bind fibrin

Function
Fibrinogen binding, platelet aggregation
Ligand/Receptor
Fibrinogen
Cell Type
Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hynes RO. 1992. Cell 69:11.
2. Shattil SJ, et al. 1985. J. Biol. Chem. 260:11107.
3. Ginsberg, et al. 1990. Blood 76:2017.
4. O’Toole TE, et al. 1991. Science 254:845.
5. Peterson JA, et al. 1998. Blood 92: 2053.

Gene ID
3647 View all products for this Gene ID 3690 View all products for this Gene ID
UniProt
View information about CD41/CD61 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/05/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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