- PAC-1 (See other available formats)
- Regulatory Status
- Other Names
- gpIIb/IIIa complex, integrin αIIbβ3
- Mouse IgM, κ
- Ave. Rating
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- Product Citations
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The gpIIb/IIIa complex, also known as integrin αIIbβ3, is expressed by megakaryocytes and platelets. It undergoes conformational changes upon activation by substances, such as ADP, thrombin, and TXA2, which subsequently reveals a ligand binding site that is specific for fibrinogen, von Willebrand factor (vWF), fibronectin, and vitronectin. This conformational change is crucial for platelet-platelet adhesion. Ligand bound gpIIb/IIIa complex promotes platelet aggregation. This complex plays an important role in hemostasis and is a target for developing therapeutic antibodies in immune thrombocytopenia.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Human platelets
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: immunoprecipitation1,2, Western blotting2 and in vitro blocking of platelet aggregation2.
The PAC-1 antibody reacts with activation-induced conformational epitope of the integrin aIIbß3. PAC-1 binding to fibrinogen is pH and Ca2+ dependent, and will not bind to platelets that have been fixed. We recommend staining in buffers free of Ethylenediaminetetraacetic acid (EDTA) at a pH of 6.5 or higher. Sodium citrate may be used as an anticoagulant for blood collection3.
(PubMed link indicates BioLegend citation)
- Shattil SJ, et al. 1987. Blood. 70:307. (IP)
- Shattil SJ, et al. 1985. J. Biol. Chem. 260:11107. (Block, IP, WB)
- Shattil SJ, et al. 1986. Blood. 68: 1224-31.
- Product Citations
AB_2563889 (BioLegend Cat. No. 362802)
- α/β transmembrane heterodimer of the ubiquitous integrin superfamily
Resting platelets; activated platelets express a conformationally modified complex that can bind fibrin
- Fibrinogen binding, platelet aggregation
- Cell Type
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Hynes RO. 1992. Cell 69:11.
2. Shattil SJ, et al. 1985. J. Biol. Chem. 260:11107.
3. Ginsberg, et al. 1990. Blood 76:2017.
4. O’Toole TE, et al. 1991. Science 254:845.
5. Peterson JA, et al. 1998. Blood 92: 2053.
- Gene ID
- 3674 View all products for this Gene ID 3690 View all products for this Gene ID
- View information about CD41/CD61 on UniProt.org
Related Pages & Pathways
Other FormatsView All CD41/CD61 Reagents Request Custom Conjugation
|Purified anti-human CD41/CD61||PAC-1||FC,IP,WB|
|FITC anti-human CD41/CD61||PAC-1||FC|
|Alexa Fluor® 647 anti-human CD41/CD61||PAC-1||FC|
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