APC anti-human CD186 (CXCR6) Antibody

Pricing & Availability
Clone
K041E5 (See other available formats)
Regulatory Status
RUO
Other Names
BONZO, STRL33, TYMSTR
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
K041E5_APC_CD186_Antibody_FC_1_061713
Human peripheral blood mononuclear cells were stimulated with IL-2 for 9 days, and then stained with CD3 FITC and CD186 (clone K041E5) APC (top) or mouse IgG2a, κ APC isotype control (bottom).
  • K041E5_APC_CD186_Antibody_FC_1_061713
    Human peripheral blood mononuclear cells were stimulated with IL-2 for 9 days, and then stained with CD3 FITC and CD186 (clone K041E5) APC (top) or mouse IgG2a, κ APC isotype control (bottom).
  • K041E5_APC_CD186_Antibody_FC_2_061113
See APC spectral data
Cat # Size Price Quantity Check Availability Save
356005 25 tests $130.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
356006 100 tests $295.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD186, also known as CXCR6, is a 39 kD, G-protein coupled chemokine receptor, with seven transmembrane-spanning regions. CXCR6 is expressed on activated and memory T cells. Its ligand is the chemokine CXCL16. CXCR6 is involved in the recruitment of cells to inflamed sites and is also a coreceptor for HIV.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
cDNA encoding human CXCR6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  2. Swadling L, et al. 2020. Cell Rep. 30:687. PubMed
  3. Stegmann K, et al. 2016. Sci Rep. 6: 26157. PubMed
  4. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
RRID
AB_2562222 (BioLegend Cat. No. 356005)
AB_2562223 (BioLegend Cat. No. 356006)

Antigen Details

Structure
GPCR, seven transmembrane-spanning regions, 39 kD
Distribution

Memory T cells, activated T cells

Function
Chemotaxis, coreceptor for HIV
Ligand/Receptor
CXCL16
Cell Type
T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Kim CH, et al. 2001. J. Clin. Invest. 107:595.
2. Nanki T, et al. 2005. Arthritis Rheum. 52:3004.
3. Van der Voort R, et al. 2005. Arthritis Rheum. 52:1381.
4. Aust G, et al. 2005. Eur. J. Endocrinol. 152:635.
5. Agostini C, et al. 2005. Am. J. Respir. Crit. Care Med. 172:1290.
6. Gaida MM, et al. 2008. Clin. Exp. Immunol. 154:216.
7. Huang Y, et al. 2008. J. Immunol. 180:2367.

Gene ID
10663 View all products for this Gene ID
UniProt
View information about CD186 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 06/18/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account