Loading...

True-Nuclear™ Transcription Factor Buffer Set

Pricing & Availability
Regulatory Status
RUO
Other Names
Transcription Factor Fixation/Permeabilization Buffer Set
Ave. Rating
Submit a Review
Product Citations
publications
Transcription_Factor_F_P_Buffer_1_ICFC_012915
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_1_ICFC_012915
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_2_ICFC_012915
Cat # Size Price Quantity Check Availability Save
424401 120 tests $165
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

BioLegend's True-Nuclear™ Transcription Factor Buffer Set has been specially formulated for intracellular staining with minimum effect on the surface fluorochrome staining.

See the advantages of True-Nuclear™ Transcription Factor Buffer Set in this brief webinar: biolegend.com/true_nuclear_webinar.


 

Caution: The True-Nuclear™ Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact. 

Product Details
Technical data sheet

Product Details

Storage & Handling
Store between 2°C and 8°C. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Prepare fresh Transcription Factor Fix working solution by diluting the 4X Fix Concentrate (1 part) with the Fix Diluent (3 parts). 1 mL of the 1X Fix working solution is needed for each tube.

Prepare a 1X working solution of the Perm Buffer by diluting the 10X Perm Buffer with distilled water. 6.5 mL of 1X Perm Buffer is needed for each sample of tube.

NOTE: The 10X Perm Buffer may have crystallization or precipitation when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is a heavy precipitation observed after dilution to 1X working solution, it can be filtered to clarify the solution.

Application Notes

Buffer Set Contents:

  • True-Nuclear™ 4X Fix Concentrate (30 mL)
  • True-Nuclear™ Fix Diluent (100 mL)
  • True-Nuclear™ 10X Perm Buffer (100 mL)

 

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells, to detect transcription factors, and to study TIGIT in our Scientific Poster Library.

Product Citations
  1. Ogawa T, et al. 2020. J Immunol. 205:907. PubMed
  2. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  3. Manso BA, et al. 2021. STAR Protoc. 2:100485. PubMed
  4. Wu HJ, et al. 2021. Nat Commun. 12:4897. PubMed
  5. Zeng N, et al. 2022. EMBO Rep. 23:e53302. PubMed
  6. Wang L, et al. 2019. Front Pharmacol. 1.342361111. PubMed
  7. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  8. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  9. Bujanover N, et al. 2021. Stem Cell Reports. 16:1884. PubMed
  10. Kim D, et al. 2021. Nanomicro Lett. 13:31. PubMed
  11. Zhang M, et al. 2020. Nat Commun. 4.707638889. PubMed
  12. Baram T, et al. 2021. Cells. 10:. PubMed
  13. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  14. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  15. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  16. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  17. Guo R, et al. 2020. Nat Microbiol. 0.938194444. PubMed
  18. van der Heiden M, et al. 2020. Scand J Immunol. e13005:. PubMed
  19. Fronik P, et al. 2021. J Med Chem. 64:12132. PubMed
  20. Webster MR, et al. 2020. Mol Cell. 77:633. PubMed
  21. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  22. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  23. Woodruff MC, et al. 2020. Nat Immunol. 1.920833333. PubMed
  24. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  25. Cortez JT, et al. 2020. Nature. 582:416. PubMed
  26. Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
  27. Noman MZ, et al. 2020. Sci Adv. 6:eaax7881. PubMed
  28. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  29. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  30. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  31. Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed
  32. Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
  33. Wei SC, et al. 2020. Cancer Discov. . PubMed
  34. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  35. Freitas JT, et al. 2021. Pigment Cell Melanoma Res. 34:1084. PubMed
  36. Adamik J, et al. 2022. Nat Commun. 13:5184. PubMed
  37. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  38. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  39. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  40. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  41. Swartz AM, et al. 2022. Curr Protoc. 2:e410. PubMed
  42. Shiao SL, et al. 2021. Cancer Cell. 39:1202. PubMed
  43. Jarjour NN, et al. 2019. Nat Immunol. 1.310416667. PubMed
  44. de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
  45. Pucella JN, et al. 2019. J Immunol. 202:1383. PubMed
  46. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  47. Woodruff M, et al. 2020. medRxiv. . PubMed
  48. Wiarda JE, et al. 2022. Life Sci Alliance. 5:. PubMed
  49. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  50. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  51. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  52. Hu HJ, et al. 2021. Cancer Immunol Immunother. 70:3541. PubMed
  53. Ye Z, et al. 2021. Nat Commun. 12:907. PubMed
  54. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  55. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  56. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  57. Kleiman E, et al. 2021. Sci Rep. 11:9561. PubMed
  58. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  59. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  60. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  61. Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
  62. Bin L, et al. 2017. APMIS. 10.1111/apm.12748. PubMed
  63. Saito E, et al. 2019. Biomaterials. 222:119432. PubMed
  64. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  65. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  66. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  67. Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed
  68. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed
  69. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  70. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  71. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  72. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  73. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  74. Datlinger P, et al. 2017. Nat Methods. 10.1038/nmeth.4177. PubMed
  75. Jarjour NN, et al. 2020. J Immunol. 204:923. PubMed
  76. Oherle K, et al. 2020. Immunity. 275:52. PubMed
  77. Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed
  78. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  79. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  80. Capelle CM, et al. 2021. Immunohorizons. 5:711. PubMed
  81. Lone W, et al. 2021. Clin Cancer Res. 27:6039. PubMed
  82. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  83. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  84. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  85. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  86. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  87. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  88. Feng Y, et al. 2019. Stem Cell Res Ther. 0.479166667. PubMed
  89. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  90. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  91. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  92. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  93. Andricovich J, et al. 2016. J Clin Invest. 126:905-20. PubMed
  94. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  95. Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed
  96. Zhao J, et al. 2021. Front Oncol. 10:621092. PubMed
  97. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  98. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  99. Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
  100. Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
  101. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
  102. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
  103. Vora AA, et al. 2021. STAR Protocols. 2(2):100565. PubMed
  104. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed

Antigen Details

Cell Type
Tregs
Biology Area
Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 05/20/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account