True-Nuclear™ Transcription Factor Buffer Set

Pricing & Availability
Other Names
Transcription Factor Fixation/Permeabilization Buffer Set
Ave. Rating
4 reviews
Product Citations
publications
Transcription_Factor_F_P_Buffer_1_ICFC_012915
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_1_ICFC_012915
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_2_ICFC_012915
Cat # Size Price Quantity Avail. Save
424401 120 tests $140
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Description

BioLegend's True-Nuclear™ Transcription Factor Buffer Set has been specially formulated for intracellular staining with minimum effect on the surface fluorochrome staining.

See the advantages of True-Nuclear™ Transcription Factor Buffer Set in this brief webinar: biolegend.com/true_nuclear_webinar.


 

Caution: The True-Nuclear™ Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact. 

Product Details
Technical data sheet

Product Details

Storage & Handling
This buffer set is guaranteed for six months. Store between 2°C and 8°C. Do not freeze.

This product has a shelf-life of 15 months or less. Please contact our technical support team for lot specific CoA and expiration date inquiries of this product.
Application

ICFC - Quality tested

Recommended Usage

Prepare fresh Transcription Factor Fix working solution by diluting the 4X Fix Concentrate (1 part) with the Fix Diluent (3 parts). 1 mL of the 1X Fix working solution is needed for each tube.

Prepare a 1X working solution of the Perm Buffer by diluting the 10X Perm Buffer with distilled water. 6.5 mL of 1X Perm Buffer is needed for each sample of tube.

NOTE: The 10X Perm Buffer may have crystallization or precipitation when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is a heavy precipitation observed after dilution to 1X working solution, it can be filtered to clarify the solution.

Application Notes

Buffer Set Contents:

  • True-Nuclear™ 4X Fix Concentrate (30 mL)
  • True-Nuclear™ Fix Diluent (100 mL)
  • True-Nuclear™ 10X Perm Buffer (100 mL)

 

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells, to detect transcription factors, and to study TIGIT in our Scientific Poster Library.

Product Citations
  1. Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
  2. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  3. Andricovich J, et al. 2016. J Clin Invest. 126:905-20. PubMed
  4. Datlinger P, et al. 2017. Nat Methods. 10.1038/nmeth.4177. PubMed
  5. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  6. Bin L, et al. 2017. APMIS. 10.1111/apm.12748. PubMed
  7. Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed
Publication Library

Antigen Details

Cell Type
Tregs
Biology Area
Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 05/26/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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