Loading...

Spark Violet™ 423 anti-human CD15 (SSEA-1) Antibody

Pricing & Availability
Clone
HI98 (See other available formats)
Regulatory Status
RUO
Workshop
IV M141
Other Names
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
Isotype
Mouse IgM, κ
Ave. Rating
Submit a Review
Product Citations
publications
HI98_SpkV423_CD15_Antibody_061322
Human peripheral blood granulocytes stained with anti-human CD15 (SSEA-1) (clone HI98) Spark Violet™ 423 (filled histogram) or mouse IgM, κ Spark Violet™ 423 isotype control (open histogram).
  • HI98_SpkV423_CD15_Antibody_061322
    Human peripheral blood granulocytes stained with anti-human CD15 (SSEA-1) (clone HI98) Spark Violet™ 423 (filled histogram) or mouse IgM, κ Spark Violet™ 423 isotype control (open histogram).
Compare all formats See Spark Violet™ 423 spectral data
Cat # Size Price Quantity Check Availability Save
301925 25 tests $160
Check Availability


Need larger quantities of this item?
Request Bulk Quote
301926 100 tests $325
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 423 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 423 has a maximum excitation of 400 nm and a maximum emission of 415 nm.

Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HI98 has been described to specifically react with 3-fucosyl-N-acetyllactosamine (3-FAL), also called X-hapten, SSEA-1, CD15, or Lewis X. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections5 and formalin-fixed paraffin-embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. van Baarsen LG, et al. 2014. Arthritis Res. Ther. 16:426. (IHC)
RRID
AB_2924521 (BioLegend Cat. No. 301925)
AB_2924521 (BioLegend Cat. No. 301926)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Adhesion
Cell Type
Embryonic Stem Cells, Eosinophils, Monocytes, Neural Stem Cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Stocks SC, et al. 1990. Biochem. J. 268:275.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/13/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD15 (SSEA-1)

  • FITC anti-human CD15 (SSEA-1)

  • PE anti-human CD15 (SSEA-1)

  • Purified anti-human CD15 (SSEA-1)

  • Alexa Fluor® 488 anti-human CD15 (SSEA-1)

  • Alexa Fluor® 594 anti-human CD15 (SSEA-1)

  • Biotin anti-human CD15 (SSEA-1)

  • Alexa Fluor® 700 anti-human CD15 (SSEA-1)

  • PE/Cyanine7 anti-human CD15 (SSEA-1)

  • PerCP/Cyanine5.5 anti-human CD15 (SSEA-1)

  • Spark Violet™ 423 anti-human CD15 (SSEA-1)

  • Spark Violet™ 538 anti-human CD15 (SSEA-1)
ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account