- Clone
- 63D3 (See other available formats)
- Other Names
- Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood monocytes were stained with CD14 (clone 63D3) Spark NIR™ 685 (filled histogram.) Open histogram represents unstained cells.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 367149 | 25 tests | $120 | ||||
| 367150 | 100 tests | $315 | ||||
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as the LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified human peripheral blood monocytes.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm. - Excitation Laser
-
Red Laser (633 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028.
- Devitt A, et al. 1998. Nature 6675:505.
- RRID
-
AB_2820022 (BioLegend Cat. No. 367149)
AB_2820023 (BioLegend Cat. No. 367150)
Antigen Details
- Structure
- GPI-linked membrane glycoprotein.
- Distribution
-
Monocytes, macrophages, dendritic cells, and granulocytes.
- Function
- LPS receptor, clearance of Gram-negative pathogens.
- Interaction
- LPS.
- Ligand/Receptor
- LPS.
- Cell Type
- Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Stocks SC, et al. 1990. Biochem. J. 268:275.
- Wright SD, et al. 1990. Science 4975:1431.
- Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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