Purified anti-Vimentin Antibody (Previously Covance catalog# PCK-594P)

Pricing & Availability
Clone
Poly29191 (See other available formats)
Other Names
CTRCT30, HEL113, epididymis luminal protein 113
Previously
Covance Catalog# PCK-594P
Isotype
Chicken Polyclonal IgY
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Product Citations
publications
1_Poly29191_Vimentin_Purified_1_IHC_021815.jpg
Immunohistochemical staining of Vimentin , using Poly29191 on formalin fixed paraffin embedded human tonsil tissue.
  • 1_Poly29191_Vimentin_Purified_1_IHC_021815.jpg
    Immunohistochemical staining of Vimentin , using Poly29191 on formalin fixed paraffin embedded human tonsil tissue.
  • 2_Poly29191_Vimentin_Purified_2_WB_021815.jpg
    Total lysates (15 µg protein) from HeLa (Human), Raw264.7 (Mouse) and UMR106 (Rat) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:5000 purified Vimentin antibody, clone Poly29191. Proteins were visualized using chemiluminescence detection by incubation with HRP Donkey anti-chicken secondary antibody (1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 3_Poly29191_Vimentin_Purified_3_WB_113018
    Total cell lysates (15 µg total protein) from Daudi (negative control), PC-3, Jurkat and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.1 µg/mL (1:5,000 dilution) of Purified anti-Vimentin Antibody, clone Poly29191, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP conjugated donkey anti-Chicken IgY antibody. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • Poly29191_Vimentin_Purified_3_ICC_021815.jpg
    Immunofluorescence of A431 cells with (A) Chicken IgG Y Isotype control (Negative, Cat. No. 402101) or (B-D) Vimentin primary antibody (Clone Poly29191). Alexa Fluor® 488 (Green) Goat anti-Chicken IgG was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/15, and (B-D) is 1/70. Concentrations for (A, B) is 1/1000, (C) is 1/2500, (D) is 1/5000.
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919101 100 µl $266
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Description

Vimentin is a protein subunit of the 10nm or intermediate filaments protein found in many kinds of mesenchymal and epithelia cells, several types of cells in tissue culture and in developing neuronal and astrocytic precursor cells in the central nervous system. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in many kinds of astrocytes), with desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, Mouse
Antibody Type
Polyclonal
Host Species
Chicken
Immunogen
This antibody was raised in chickens against purified recombinant vimentin.
Formulation
Phosphate-buffered solution, containing 10 mM sodium azide.
Preparation
The antibody is an IgY preparation.
Concentration
~20 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB, ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a dilution of 1:5000 is suggested. For Western blotting, the suggested use of this reagent is 1:2000 - 1:8000 dilution. For immunocytochemistry, a dilution range of 1:1000 - 1:5000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunofluorescence (IF) and immunohistochemistry (IHC). This antibody does not bind to protein A or protein G.

 

Predicted MW is ~54 kD

Application References

(PubMed link indicates BioLegend citation)
  1. Herrmann H, et. al. 1989. Development 105:279. (IF, ICC, IHC, WB)
  2. KivelS T, et. al. 1992. J. Histochem. Cytochem. 40:1517. (IHC)
  3. Stathopoulos, et. al. 1989. J. Histochem. Cytochem. 37:1363. (IHC)
Product Citations
  1. Robert A, et al. 2015. Proc Natl Acad Sci U S A. 112: 3505 - 3514. PubMed
  2. Lowery J, et al. 2016. Mol Biol Cell. 27: 608 - 616. PubMed
  3. Tiwari A, et al. 2016. Sci Rep. 6:38681. PubMed
  4. Lee DH, et al. 2019. PLoS One. 14:e0211153. PubMed
  5. Almada P, et al. 2019. Nat Commun. 10:1223. PubMed
Publication Library
RRID
AB_2565208 (BioLegend Cat. No. 919101)

Antigen Details

Cell Type
Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Intermediate Filaments
Gene ID
7431 View all products for this Gene ID
UniProt
View information about Vimentin on UniProt.org

Related FAQs

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Go To Top Version: 7    Revision Date: 12/13/2018

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