Purified anti-Tyrosine Hydroxylase Antibody (Previously Covance catalog# MMS-5210, MMS-5210-100, MMS5210)

Pricing & Availability
Clone
2/40/15 (See other available formats)
Other Names
Tyrosine 3-monooxygenase, tyrosine 3-hydroxylase
Previously
Covance Catalog# MMS-5210, MMS-5210-100, MMS5210
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
24015_pure_Tyrosine-Hydroxylase_Antibody_1_030618
IHC staining of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded mouse (A) and (B) rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissues were incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • 24015_pure_Tyrosine-Hydroxylase_Antibody_1_030618
    IHC staining of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded mouse (A) and (B) rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissues were incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • 24015_pure_Tyrosine-Hydroxylase_Antibody_2_030618
    IHC staining of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 24015_pure_Tyrosine-Hydroxylase_Antibody_3_030618
    IHC staining of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 24015_pure_Tyrosine-Hydroxylase_Antibody_4_030618
    IHC staining of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 24015_pure_Tyrosine-Hydroxylase_Antibody_5_030618
    IHC staining of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 24015_pure_Tyrosine-Hydroxylase_Antibody_6_030618
    Western blot of purified anti-Tyrosine Hydroxylase antibody (clone 2/40/15). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain lysate; Lane 3: 30 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
818002 25 µl $90
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818001 100 µl $234
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Description

Tyrosine hydroxylase, also known as tyrosine 3-monooxygenase, is the enzyme that converts amino acid L-Tyrosine to L-3, 4-Dihydroxyphenylalanine (L-DOPA).  L-DOPA is a precursor to dopamine, which is then converted to norepinephrine and epinephrine (adrenaline).  Dopamine, norepinephrine and epinephrine are all important neurotransmitters.  Alterations in the activity of tyrosine hydroxylase may be involved in a number of neurological diseases such as Parkinson's and schizophrenia.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against purified Tyrosine Hydroxylase from rat Pheochromocytoma tumor.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 1.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).

This antibody is less reactive in human tissue in Western Blot.

RRID
AB_2734573 (BioLegend Cat. No. 818002)
AB_2564816 (BioLegend Cat. No. 818001)

Antigen Details

Structure
Expected MW: 65 kD
Cell Type
Dopaminergic Neurons, Neural Stem Cells, Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family
Enzymes and Regulators, Presynaptic proteins
Gene ID
25085 View all products for this Gene ID
UniProt
View information about Tyrosine Hydroxylase on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 03/06/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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