Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P)

Pricing & Availability
Clone
Poly18020 (See other available formats)
Other Names
CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, TUBB3, tubulin beta-4 chain, class III beta-tubulin
Previously
Covance Catalog# PRB-435P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
1 reviews
Product Citations
publications
A_Poly18020_PURE_Tubulin_beta3_IHCP_1_032918
IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded rat cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A_Poly18020_PURE_Tubulin_beta3_IHCP_1_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded rat cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B_Poly18020_PURE_Tubulin_beta3_IHCP_2_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • C_Poly18020_PURE_Tubulin_beta3_IHCP_3_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly18020_PURE_Tubulin_beta3_WB_021915.jpg
    Western blot of purified anti-Tubulin β-3 (TUBB3) antibody (poly18020). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 0.1 µg/mL of poly18020 or Rabbit IgG overnight at 4°C, followed by incubation with HRP-labeled anti-rabbit IgG (Cat. No. 410406). Enhanced chemiluminescence was used as the detection system.
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802001 100 µl $369
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Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against microtubules derived from rat brain.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Validated
ICC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 0.5 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunocytochemistry (ICC).

This antibody is well characterized and highly reactive to neuron specific Class III β-Tubulin (βIII), but does not identify β-tubulin found in glial cells. This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Feuer R, et al. 2005. J. Neurosci. 25(9):2434-44.
  2. Mozzetti S, et al. 2005. Clin. Cancer Res. 11(1):298-305.
  3. Feuer R, et al. 2003. Am. J. Pathol. 163(4):1379-93.
  4. Zhang XM, Yang XJ. 2001. Development. 128(6):943-57.
  5. Ma M, et al. 2012. Neurobiol Dis. 56:34. (WB) PubMed
  6. Tseung G, et al. 2011. J. Virol. 85:5718. (IF) PubMed
  7. Shimada IS, et al. 2012. J Neurosci. 32:7926. (IHC) PubMed
  8. Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed
Product Citations
  1. Macpherson P, et al. 2015. Development. 142: 4038 - 4048. PubMed
  2. Ferguson M, et al. 2013. Neurobiol Dis. 56:34-46. PubMed
  3. Tsueng G, et al. 2011. J Virol. 85:5718-5732. PubMed
  4. Flores-Otero J, et al. 2007. J Neurosci. 27:14023-14034. PubMed
  5. Shimada I, et al. 2012. J Neurosci. 32:7926-7940. PubMed
  6. Chow A, Morshead C 2016. Stem Cell Res. 16:79-87. PubMed
  7. Yau K, et al. 2016. J Neurosci. 36: 1071 - 1085. PubMed
  8. Cui Q, et al. 2016. Nat Commun. 7:10637. PubMed
  9. Vicidomini C, et al. 2016. Mol Psychiatry. 10.1038/mp.2016.30. PubMed
  10. Otani T, et al. 2016. Cell Stem Cell. 18: 467-480. PubMed
  11. Anastasiadou S, Knöll B 2016. Exp Neurol. 279:243-260. PubMed
  12. Son M, et al. 2016. Exp Mol Med. 48: 232. PubMed
  13. Baleriola J, et al. 2016. Sci Rep. 6: 25928. PubMed
  14. Krishnan V, et al. 2016. J Neuropathol Exp Neurol. 75: 464 - 478. PubMed
  15. Tian E, et al. 2016. Cell Rep. 16: 781-792. PubMed
  16. Lin P, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G412 - G422. PubMed
  17. Zhang L, et al. 2016. Neuroscience. 337:88-97. PubMed
  18. Watanabe S, et al. 2016. EMBO Mol Med. 8(12):1421-1437. PubMed
  19. Brykczynska U, et al. 2016. Stem Cell Reports. 7:1059-1071. PubMed
  20. Boland M, et al. 2017. Brain. 10.1093/brain/aww357. PubMed
  21. Tseng K, et al. 2017. eNeuro. 10.1523/ENEURO.0214-17.2017. PubMed
  22. Benoy V, et al. 2018. Brain. 10.1093/brain/awx375. PubMed
  23. Xie Y, et al. 2018. Stem Cell Res. 9:2010. PubMed
  24. Carlin D, et al. 2018. Eneuro. 5. PubMed
  25. Qiang L 2018. Current biology : CB. 28:2181. PubMed
  26. Zahr SK 2018. Neuron. 97:520. PubMed
  27. Ou J 2018. Cell. 173:851. PubMed
  28. Kawakami Y 2018. The Journal of neuroscience : the official journal of the Society for Neuroscience. n/a. PubMed
  29. Jean-Louis T, et al. 2018. Neurobiol Aging. 62:130. PubMed
  30. Hung COY, et al. 2018. Cell Rep. 25:3647. PubMed
  31. Toda T, et al. 2017. Cell Stem Cell. 21:618. PubMed
  32. Makar T, et al. 2018. Stem Cell Res Ther. 9:127. PubMed
  33. Shang Y, et al. 2018. J Neurosci. 38:4006. PubMed
  34. Jeon H, et al. 2018. J Clin Med. 7:. PubMed
  35. Lee J, et al. 2018. J Clin Med. 7:. PubMed
Publication Library
RRID
AB_2564645 (BioLegend Cat. No. 802001)

Antigen Details

Cell Type
Neural Stem Cells, Mature Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Gene ID
10381 View all products for this Gene ID
UniProt
View information about Tubulin beta-3 (TUBB3) on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 03/29/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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