Purified anti-Tubulin-α Antibody

Pricing & Availability
Clone
10D8 (See other available formats)
Other Names
α-tubulin, Tubulin, alpha-ubiquitous chain
Isotype
Mouse IgM, κ
Ave. Rating
1 reviews
Product Citations
publications
10D8_PURE_Tubulin-a_Antibody_WB_113017
Whole cell lysates (15 µg protein) from HeLa (lane 1), Jurkat (lane 2) and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (0.5 µg /mL) and 1:2500 (0.2 µg /mL) purified anti-Tubulin-α antibody (clone 10D8). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse IgM secondary antibody. 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). 
  • 10D8_PURE_Tubulin-a_Antibody_WB_113017
    Whole cell lysates (15 µg protein) from HeLa (lane 1), Jurkat (lane 2) and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (0.5 µg /mL) and 1:2500 (0.2 µg /mL) purified anti-Tubulin-α antibody (clone 10D8). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse IgM secondary antibody. 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). 
Cat # Size Price Quantity Avail. Save
627901 25 µg $85
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627902 100 µg $185
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Description

Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, All Species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human α-tubulin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
This antibody is at >85% purity.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB, IF

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescent staining applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Chou HYE, et al. 2006. J. Biol. Chem. 281:15201.
Product Citations
  1. Gärtner K, et al. 2018. Cancer Med. 11:39. PubMed
Publication Library
RRID
AB_439760 (BioLegend Cat. No. 627901)
AB_439761 (BioLegend Cat. No. 627902)

Antigen Details

Structure
Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.
Distribution

Ubiquitous

Function
Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site
Interaction
RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A
Modification
Phosphorylation, acetylation
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Antigen References

1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738.
2. Miller FD, et al. 1987. J. Cell Biol. 105:3065.
3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96.

Gene ID
84790 View all products for this Gene ID
UniProt
View information about Tubulin-alpha on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 11/30/2017

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