Alexa Fluor® 488 anti-Tubulin-α Antibody

Pricing & Availability
Clone
10D8 (See other available formats)
Other Names
α-tubulin, Tubulin, alpha-ubiquitous chain
Isotype
Mouse IgM, κ
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Product Citations
publications
10D8_AF488_Tubulin-alpha_Antibody_IF_050316
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 1 µg/ml of Alexa Fluor® 488 anti-Tubulin-α (clone 10D8) antibody (green) in 5% FBS for two hours in room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with 60X objective.
  • 10D8_AF488_Tubulin-alpha_Antibody_IF_050316
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 1 µg/ml of Alexa Fluor® 488 anti-Tubulin-α (clone 10D8) antibody (green) in 5% FBS for two hours in room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with 60X objective.
See Alexa Fluor® 488 spectral data See high resolution IF data...
Cat # Size Price Quantity Avail. Save
627905 25 µg $150
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627906 100 µg $295
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Description

Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, All Species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human α-tubulin
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was conjugated with Alexa Fluor® 488 under optimal conditions, and is at >85% purity.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IF - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescent staining applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Chou HYE, et al. 2006. J. Biol. Chem. 281:15201.
RRID
AB_893643 (BioLegend Cat. No. 627905)
AB_893642 (BioLegend Cat. No. 627906)

Antigen Details

Structure
Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.
Distribution

Ubiquitous

Function
Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site
Interaction
RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A
Modification
Phosphorylation, acetylation
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Antigen References

1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738.
2. Miller FD, et al. 1987. J. Cell Biol. 105:3065.
3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96.

Gene ID
84790 View all products for this Gene ID
UniProt
View information about Tubulin-alpha on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 05/03/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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