Purified anti-Pax-6 Antibody (Previously Covance catalog# PRB-278P)

Pricing & Availability
Clone
Poly19013 (See other available formats)
Other Names
Paired box protein Pax-6, oculorhombin, aniridia type II protein, Sey, Protein eyeless, PRB-278P-100
Previously
Covance Catalog# PRB-278P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
3 reviews
Product Citations
publications
1_Poly19013_PURE_Pax-6_Antibody_1_WB_112817
Western blot analysis of cell lysates from 293T (human), UMR106 (rat) and Raw264.7 (mouse) using Pax-6 rabbit primary antibody (Clone Poly19013, 1:2000 dilution, 1 µg/ml) and HRP Donkey anti-rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). GAPDH (Cat. No. 919501) was used as a loading control (1:2500 dilution).
  • 1_Poly19013_PURE_Pax-6_Antibody_1_WB_112817
    Western blot analysis of cell lysates from 293T (human), UMR106 (rat) and Raw264.7 (mouse) using Pax-6 rabbit primary antibody (Clone Poly19013, 1:2000 dilution, 1 µg/ml) and HRP Donkey anti-rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). GAPDH (Cat. No. 919501) was used as a loading control (1:2500 dilution).
  • 2_Purified_Pax-6_Antibody_IHC_1_021315
    Strong nuclear staining using PAX-6 on formalin-fixed paraffin-embedded human epididymis tissue.
  • 3_Purified_Pax-6_Antibody_IHC_2_021315
    Strong nuclear staining using PAX-6 on formalin-fixed paraffin-embedded mouse brain tissue.
  • 4_Poly19013_PURE_Pax-6_Antibody_2_WB_122017
    Western blot analysis of 15 µg protein from PC-3, HeLa and U87-MG cells. Lysates were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:2000 (1 µg/ml) purified anti-Pax-6 antibody, clone Poly19013. Proteins were visualized using chemiluminescence detection by incubation with HRP Donkey anti-rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 5_Milad_Riazifar
    Frozen human iPSC derived neural rosettes stained with purified anti-Pax-6 (red, clone Poly19013) and ZO-1 (green). Image generously submitted to the 2017 Cell Life Imaging Competition by Milad Riazifar from University of California, Irvine.
Cat # Size Price Quantity Avail. Save
901301 100 µl $351.00
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Description

Pax6 is a transcription factor present during embryonic development. The encoded protein contains two different binding sites that are known to bind DNA and function as a regulator of gene transcription. It is a key regulatory gene of eye and brain development. Within the brain, the protein is involved in development of specialized cells that process smell. Pax-6 acts as a critical gene for the development of eyes and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. Pax6 serves as a regulator in the coordination and pattern formation required for differentiation and proliferation to successfully take place, ensuring that the processes of neurogenesis and oculogenesis are carried out successfully. As a transcription factor, Pax6 acts at the molecular level in the signaling and formation of the central nervous system. The characteristic paired DNA binding domain of Pax6 utilizes two DNA-binding domains, the paired domain (PD), and the paired-type homeodomain (HD). These domains function separately. An example of this lies in HD’s regulatory involvement in the formation of the lens and retina throughout oculogenesis contrasted by the molecular mechanisms of control exhibited on the patterns of neurogenesis in brain development by PD. The HD and PD domains act in close coordination, giving Pax6 its multifunctional nature in directing molecular signaling in formation of the CNS.

The vertebrate PAX6 locus encodes at least three different protein isoforms, these being the canonical PAX6, PAX6(5a), and PAX6(ΔPD). The canonical PAX6 protein contains an N-terminal paired domain, connected by a linker region to a paired-type homeodomain, and a proline/serine/threonine (P/S/T)-rich C-terminal domain. The paired domain and paired-type homeodomain each have DNA binding activities, while the P/S/T-rich domain possesses a transactivation function. PAX6(5a) is a product of the alternatively spliced exon 5a resulting in a 14 residue insertion in the paired domain which alters the specificity of this DNA binding activity. The nucleotide sequence corresponding to the linker region encodes a set of three alternative translation start codons from which the third PAX6 isoform originates. Collectively known as the PAX6(ΔPD) or pairedless isoforms, these three gene products all lack a paired domain. The pairedless proteins possess molecular weights of 43, 33, or 32kDa, depending on the particular start codon used. PAX6 transactivation function is attributed to the variable length C-terminal P/S/T-rich domain which stretches to 153 residues in human and mouse proteins.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Mammalian
Host Species
Rabbit
Immunogen
This antibody was generated against the peptide (QVPGSEPDMSQYWPRLQ) derived from the C-terminus of the mouse Pax-6 protein.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC - Validated
IF - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting and immunohistochemistry.

The optimal working dilution should be determined for each specific assay condition.
Western blot: 1 µg/ml (1:2000)
IHC: 1:50-1:100
IF: 1:300

Tissue Sections: paraffin-embedded tissues, frozen sections
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as citrate buffer retrieval solution (928501, 928601).
Incubation: 60 minutes at room temperature

Application Notes

This antibody weakly reacts with rat. It is effective in immunoblotting (WB), immunohistochemistry (IHC), immunofluorescence (IF) and staining of tissue sections.

Pax-6 has 2 ISOFORMS in human at 46.6 and 48.2 Kd. This antibody recognizes both.

Positive Control: human brain, epididymis

The sequence is highly conserved among Pax-6 of various species. The antibody was subsequently purified on a Protein A column and is useful in studying brain, neuronal and olfactory development in higher eukaryotes.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References

(PubMed link indicates BioLegend citation)
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Publication Library
RRID
AB_2565003 (BioLegend Cat. No. 901301)

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