- Poly19013 (See other available formats)
- Other Names
- Paired box protein Pax-6, oculorhombin, aniridia type II protein, Sey, Protein eyeless, PRB-278P-100
Covance Catalog# PRB-278P
- Rabbit Polyclonal IgG
- Ave. Rating
- 3 reviews
- Product Citations
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Pax6 is a transcription factor present during embryonic development. The encoded protein contains two different binding sites that are known to bind DNA and function as a regulator of gene transcription. It is a key regulatory gene of eye and brain development. Within the brain, the protein is involved in development of specialized cells that process smell. Pax-6 acts as a critical gene for the development of eyes and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. Pax6 serves as a regulator in the coordination and pattern formation required for differentiation and proliferation to successfully take place, ensuring that the processes of neurogenesis and oculogenesis are carried out successfully. As a transcription factor, Pax6 acts at the molecular level in the signaling and formation of the central nervous system. The characteristic paired DNA binding domain of Pax6 utilizes two DNA-binding domains, the paired domain (PD), and the paired-type homeodomain (HD). These domains function separately. An example of this lies in HD’s regulatory involvement in the formation of the lens and retina throughout oculogenesis contrasted by the molecular mechanisms of control exhibited on the patterns of neurogenesis in brain development by PD. The HD and PD domains act in close coordination, giving Pax6 its multifunctional nature in directing molecular signaling in formation of the CNS.
The vertebrate PAX6 locus encodes at least three different protein isoforms, these being the canonical PAX6, PAX6(5a), and PAX6(ΔPD). The canonical PAX6 protein contains an N-terminal paired domain, connected by a linker region to a paired-type homeodomain, and a proline/serine/threonine (P/S/T)-rich C-terminal domain. The paired domain and paired-type homeodomain each have DNA binding activities, while the P/S/T-rich domain possesses a transactivation function. PAX6(5a) is a product of the alternatively spliced exon 5a resulting in a 14 residue insertion in the paired domain which alters the specificity of this DNA binding activity. The nucleotide sequence corresponding to the linker region encodes a set of three alternative translation start codons from which the third PAX6 isoform originates. Collectively known as the PAX6(ΔPD) or pairedless isoforms, these three gene products all lack a paired domain. The pairedless proteins possess molecular weights of 43, 33, or 32kDa, depending on the particular start codon used. PAX6 transactivation function is attributed to the variable length C-terminal P/S/T-rich domain which stretches to 153 residues in human and mouse proteins.
- Human, Mouse, Rat
- Antibody Type
- Host Species
- This antibody was generated against the peptide (QVPGSEPDMSQYWPRLQ) derived from the C-terminus of the mouse Pax-6 protein.
- Phosphate-buffered solution + 0.03% Thimerosal.
- The antibody was purified by affinity chromatography.
- 2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
WB, IHC-P - Quality tested
IF, IHC-F - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting and formalin-fixed paraffin-embedded immunohistochemical staining. For Western blotting, the suggested use of this reagent is 1.0 µg/ml (1:2000). For immunohistochemistry, a dilution of 1:50 - 1:100 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody weakly reacts with rat. It has also shown reactivity with GFP and is therefore not recommended for use in GFP-expressing systems (as determined by in-house testing).
Pax-6 has two isoforms in human and mice at 46.6 and 48.2 Kd. This antibody recognizes both. The sequence is highly conserved among Pax-6 of various species. The antibody was subsequently purified on a Protein A column and is useful in studying brain, neuronal and olfactory development in higher eukaryotes.
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
(PubMed link indicates BioLegend citation)
- Tang K, et al. 2012. Development. 139:1630. (IF) PubMed
- Bandah D, et al. 2007. Invest. Ophthalmol. Vis. Sci. 48:2503. (WB) PubMed
- Marquardt T, et al. 2001. Cell. 105:43.
- Yamamoto Y, Jeffery WR. 2000. Science. 289:631
- Ashery-Padan R, et al. 2000. Genes Dev. 14:2701.
- Osumi N, et al. 1997. Development. 124:2961.
- Koroma BM, et al. 1997. Invest. Ophthalmol Vis Sci. 38:108.
- Davis JA, Reed RR. 1996. J Neurosci. 16:5082. (IHC)
- Quadrato G, et al. Nature. 545:48. (IF) PubMed
- Product Citations
AB_2749901 (BioLegend Cat. No. 901302)
AB_2565003 (BioLegend Cat. No. 901301)
- Neuroscience Cell Markers
- Cell Type Markers
- Neural Stem Cell
- Transcription Regulators
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