FITC anti-human CD45 Antibody

Pricing & Availability
Clone
HI30 (See other available formats)
Workshop
IV N816
Other Names
LCA, T200
Isotype
Mouse IgG1, κ
Ave. Rating
3 reviews
Product Citations
publications
Hi30
Human peripheral blood lymphocytes, monocytes and granulocytes stained with HI30 FITC
  • Hi30
    Human peripheral blood lymphocytes, monocytes and granulocytes stained with HI30 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
304005 25 tests $30
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304006 100 tests $65
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304054 100 µg $120
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304038 500 tests $295
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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
µg sizes: 0.5 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, and Western blotting3.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
Product Citations
  1. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. PubMed
  2. Hsieh J, et al. 2013. Nucleic Acids Res. 41:9753. PubMed
  3. Didigu C, et al. 2014. Blood. 123:61. PubMed
  4. Zhang B, et al. 2013. PLoS One. 8:57114. PubMed
  5. Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed
  6. Skrbo N, et al. 2014. PLoS One. 9:113278. PubMed
  7. Saunders L, et al. 2015. Sci Transl Med. 7: 302ra136. PubMed
  8. Grove K, et al. 2016. PLoS One. 11: 0145961. PubMed
  9. Pabst C, et al. 2016. Blood. 127: 2018-2027. PubMed
  10. Genbacev O, et al. 2016. Hum Reprod. 31: 1300 - 1314. PubMed
  11. Yuan Z, et al. 2016. J Virol. 90: 7728 - 7739. PubMed
  12. S Tzeng 2016. J Vis Exp. 118:e54582. PubMed
  13. Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed
  14. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  15. Yan Y, et al. 2018. JCI Insight. 3. PubMed
  16. Wu L, et al. 2018. Oncol Lett. 15:9507. PubMed
  17. Ding L, et al. 2018. Cell Rep. 25:2972. PubMed
  18. Rousset F, et al. 2018. Mol Ther Nucleic Acids. 14:351. PubMed
  19. Niu C, et al. 2017. Mol Med Rep. 16:4879. PubMed
  20. Sprouse ML, et al. 2019. Int J Mol Sci. 20:8. PubMed
Publication Library
RRID
AB_314393 (BioLegend Cat. No. 304005)
AB_314394 (BioLegend Cat. No. 304006)
AB_2564154 (BioLegend Cat. No. 304054)
AB_2562050 (BioLegend Cat. No. 304038)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 08/19/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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