Purified anti-mouse IgMb Antibody

Pricing & Availability
Clone
AF6-78 (See other available formats)
Other Names
Igh-6b
Isotype
Mouse (BALB/c) IgG1, κ
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Product Citations
publications
AF6-78_Pure_091907
C57BL/6 mouse splenocytes stained with purified AF6-78, followd by anti-mouse IgG1 FITC
  • AF6-78_Pure_091907
    C57BL/6 mouse splenocytes stained with purified AF6-78, followd by anti-mouse IgG1 FITC
Cat # Size Price Quantity Avail. Save
406202 500 µg $220
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Description

This monoclonal antibody was raised by immunization with C57BL/10 mouse splenocytes. It specifically reacts with IgM of Igh-Cb and the related haplotypes (d and n), but not with the free μ heavy chain of IgM. Some cross-reaction with IgM of Igh-Ce can be detected with this antibody.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ELISA - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, depletion of lymphocytes.

Application References

(PubMed link indicates BioLegend citation)
  1. Stall AM, et al. 1984. J. Immunol. 132:787.
  2. Velardi A, et al. 1984. J. Immunol. 133:2098.
RRID
AB_315035 (BioLegend Cat. No. 406202)

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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