FITC anti-mouse IgMb Antibody

Pricing & Availability
Clone
AF6-78 (See other available formats)
Other Names
Igh-6b
Isotype
Mouse (BALB/c) IgG1, κ
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Product Citations
publications
AF6-78_FITC_091907
C57BL/6 mouse splenocytes stained with AF6-78 FITC
  • AF6-78_FITC_091907
    C57BL/6 mouse splenocytes stained with AF6-78 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
406205 50 µg $90
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406206 500 µg $280
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Description

This monoclonal antibody was raised by immunization with C57BL/10 mouse splenocytes. It specifically reacts with IgM of Igh-Cb and the related haplotypes (d and n), but not with the free μ heavy chain of IgM. Some cross-reaction with IgM of Igh-Ce can be detected with this antibody.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, depletion of lymphocytes.

Application References

(PubMed link indicates BioLegend citation)
  1. Stall AM, et al. 1984. J. Immunol. 132:787.
  2. Velardi A, et al. 1984. J. Immunol. 133:2098.
  3. Duong BH, et al. 2010. J. Immunol. 184:4143. PubMed
  4. Aschermann S, et al. 2013. PNAS. 110:19042. PubMed
Product Citations
  1. Duong B, et al. 2010. J Immunol. 184:4143. PubMed
  2. Aschermann S, et al. 2013. Proc Natl Acad Sci U S A. 110:19042. PubMed
  3. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  4. Firl DJ 2018. eLife. 7: e33051. PubMed
Publication Library
RRID
AB_315038 (BioLegend Cat. No. 406205)
AB_315039 (BioLegend Cat. No. 406206)

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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