Purified anti-mouse H-2Kd Antibody

Pricing & Availability
Clone
SF1-1.1 (See other available formats)
Other Names
MHC class I
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
SF1-1dot1_Pure_A_020708
Balb/c mouse splenocytes stained with purified SF1-1.1, followed by anti-mouse IgG2a FITC
  • SF1-1dot1_Pure_A_020708
    Balb/c mouse splenocytes stained with purified SF1-1.1, followed by anti-mouse IgG2a FITC
  • SF1-1dot1_Pure_B_020708
    Balb/c mouse splenocytes stained with purified SF1-1.1, followed by anti-mouse IgG2a FITC
Cat # Size Price Quantity Avail. Save
116601 50 µg $65
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Description

The SF1-1.1 antibody reacts with the H-2Kd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd haplotype. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse d haplotype, Weakly cross-reacts with H-2k haplotype
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c Mouse cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The SF1-1.1 antibody is weakly cross-reactive with H-2k but does not cross-react with other haplotypes (b, j, p, q, s, v). Clone SF1-1.1 recognizes the α3 domain of Kd.

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4 and Western blotting2.

Application References

(PubMed link indicates BioLegend citation)
  1. Noun G, et al. 1996. J. Immunol. 157:2455. (IP)
  2. Abastado JP, et al. 1993. J. Immunol. 151:3569. (WB)
  3. Bashuda H, et al. 1997. Transplantation 63:113.
  4. Sester M, et al. 2000. J. Biol. Chem. 34:113. (IP)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169. (FC)
  6. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
  7. Norian L, et al. 2004. J. Immunol.. 173:835. PubMed
  8. Delon J, et al. 1998. Immunity 9:467.
Product Citations
  1. Allen L 2004. J Immunol. 173:835. PubMed
  2. Echeverria GV, et al. 2018. Nat Commun. 9:5079. PubMed
Publication Library
RRID
AB_313736 (BioLegend Cat. No. 116601)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kd haplotype

Function
Antigen presentation
Ligand Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
3. York I, et al. 1996. Annu. Rev. Immunol. 14:369.

Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kd on UniProt.org

Related FAQs

There are no FAQs for this product.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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