- Clone
- S17014E (See other available formats)
- Other Names
- Fcgr3, Fc receptor, IgG, low affinity III
- Isotype
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
- publications
-
C57BL/6 mouse splenocytes were stained with NK1.1 Brilliant Violet 785™ and purified CD16 (clone S17014E, left) or purified rat IgG2a, κ (right), followed by anti-rat IgG Alexa Fluor® 647. -
C57BL/6 mouse bone marrow were stained with purified CD16 (clone S17014E, filled histogram) or purified rat IgG2a, κ (open histogram), followed by anti-rat IgG Alexa Fluor® 647. Data shown was gated on the myeloid cell population. -
RBL-1 cells transfected with mouse A) CD16, B) CD32 or C) CD64 were stained with the following antibodies: Anti-mouse CD16 clone S17014E, Anti-mouse CD32 clone S17012B and Anti-mouse CD64 clone S18017D to confirm their specificity (filled histograms). Open histograms shows the corresponding isotype control.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 158002 | 500 µg | $230 | ||||
CD16 also know as Fcgr3 is a 50-65 kD type I transmembrane protein, member of the Fc gamma receptor family and Ig superfamily. CD16 is expressed on most myeloid cells including monocytes, macrophages, dendritic cells, and is also expressed by NK cells and NKT cells. CD16 is involved in cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC); its ligands are IgG1, IgG2a and IgG2b.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD16 - transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Clone S17014E does not cross-react with CD32 or CD64. - RRID
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AB_2814096 (BioLegend Cat. No. 158002)
Antigen Details
- Structure
- Type I transmembrane protein, 50-65 kDa; member of the Fc gamma receptor family and Ig superfamily
- Distribution
-
Expressed on most myeloid cells including monocytes, macrophages, dendritic cells; also on NK cells and NKT cells
- Function
- Fc receptor, involved in cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
- Ligand/Receptor
- IgG1, IgG2a and IgG2b
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
- Nimmerjahn F1 & Ravetch JV. 2008. Nat Rev Immunol. 8(1):34-47.
- Biburger M & Nimmerjahn F. 2012. Immunol Lett. 143(1):53-9.
- Arase N, et al. 2003. J Immunol. 170:3054.
- Gene ID
- 14131 View all products for this Gene ID
- UniProt
- View information about CD16 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
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