C57BL/6 mouse bone marrow cells were stained with CD16 PE/Dazzle™ 594 (clone S17014E) (filled histogram) or rat IgG2a, κ PE/Dazzle™ 594 (open histogram). Data shown were gated on the myeloid cell population.
C57BL/6 mouse bone marrow cells were stained with CD16 PE/Dazzle™ 594 (clone S17014E) (filled histogram) or rat IgG2a, κ PE/Dazzle™ 594 (open histogram). Data shown were gated on the myeloid cell population.
RBL-1 cells transfected with mouse A) CD16, B) CD32 or C) CD64 were stained with the following antibodies: Anti-mouse CD16 clone S17014E, Anti-mouse CD32 clone S17012B and Anti-mouse CD64 clone S18017D to confirm their specificity (filled histograms). Open histograms shows the corresponding isotype control.
CD16 also know as Fcgr3 is a 50-65 kD type I transmembrane protein, member of the Fc gamma receptor family and Ig superfamily. CD16 is expressed on most myeloid cells including monocytes, macrophages, dendritic cells, and is also expressed by NK cells and NKT cells. CD16 is involved in cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC); its ligands are IgG1, IgG2a and IgG2b.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Clone S17014E does not cross-react with CD32 or CD64.
Excitation Laser
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
