- Clone
- N418 (See other available formats)
- Other Names
- αX integrin, integrin αX chain, CR4, p150, ITGAX
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- 0 reviews

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C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and purified N418 (top) or purified Armenian hamster IgG isotype control (bottom), followed by anti-Armenian hamster IgG FITC -
Cat # | Size | Price | Quantity Avail. | Save | ||
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117301 | 50 µg | $30.00 | ||||
117302 | 500 µg | $120.00 |
CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.
Product DetailsProduct Details
- Reactivity
- Mouse
- Host Species
- Armenian Hamster
- Immunogen
- Mouse spleen dendritic cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Validated
IP, IHC - Reported in the literature - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, and immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10).
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Application References
(PubMed link indicates BioLegend citation) -
- Granucci F, et al. 1997. J. Immunol. 159:1794.
- Stokes RW, et al. 1998. J. Immunol. 160:5514.
- Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
- Ma XT, et al. 2006. Cancer Research 66:1169.
- Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
- Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
- Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
- Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
- You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
- Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
- Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
- Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
- Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
- Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
- Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
- Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
- Wikstrom M, et al. 2006. J Immunol. 177:913. PubMed
- Benson M, et al. 2007. J Exp Med. 204:1765. PubMed
- Pericolini E, et al. 2008. J Leukoc Biol. 83:1286. PubMed
- Osterholzer J, et al. 2009. J Immunol. 183:8044. PubMed
- Fahlén-Yrlid L, et al. 2009. J Immunol. 183:5032. PubMed
- Eisenach P, et al. 2010. J Cell Sci. 123:4182. PubMed
- Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
- Leppin K, et al. 2014. Invest Ophthalmol Vis Sci . 55:3603. PubMed
- Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
- Aparicio-Domingo P, et al. 2015. J Exp Med. 101084/jm20150318. PubMed
- Molica F,et al. 2017. Sci Rep.. 10.1038/s41598-017-14130-4. PubMed
- Lechner AJ, et al. 2017. Cell Stem Cell. 21:120. PubMed
- Caucheteux SM, et al. 2017. Mucosal Immunol. 10:361. PubMed
- Cheng M, et al. 2017. Sci Rep. 7:7471. PubMed
- RRID
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AB_313770 (BioLegend Cat. No. 117301)
AB_313771 (BioLegend Cat. No. 117302)
Antigen Details
- Structure
- Integrin α-chain, associates with integrin β2 (CD18), 150 kD
- Distribution
- Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells
- Function
- Cellular adhesion
- Ligand Receptor
- iC3b, fibrinogen
- Antigen References
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1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780. - Gene ID
- 16411 View all products for this Gene ID
- UniProt
- View information about CD11c on UniProt.org
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