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Purified anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
N418_A_Purified_Pos_071006.jpg
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and purified N418 (top) or purified Armenian hamster IgG isotype control (bottom), followed by anti-Armenian hamster IgG FITC
  • N418_A_Purified_Pos_071006.jpg
    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and purified N418 (top) or purified Armenian hamster IgG isotype control (bottom), followed by anti-Armenian hamster IgG FITC
  • N418_B_Purified_Neg_071006.jpg
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117301 50 µg $33
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117302 500 µg $132
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Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
IP, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Application References

(PubMed link indicates BioLegend citation)
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  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  2. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
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  4. Sutton C, et al. 2017. Nat Commun.. 10.1038/s41467-017-02111-0. PubMed
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  8. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  9. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
  10. Fu H, et al. 2019. Front Immunol. 10:271. PubMed
  11. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  12. Zhang Z, et al. 2018. Nat Med. 24:617. PubMed
  13. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  14. Simmons S et al. 2019. Elife. 8 pii: e41239. PubMed
  15. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  16. Goltsev Y et al. 2018. Cell. 174(4):968-981 . PubMed
  17. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  18. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  19. Danne C et al. 2017. Cell host & microbe. 22(6):733-745 . PubMed
  20. Kitai Y, et al. 2017. J Immunol. 198:1649. PubMed
  21. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  22. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  23. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  24. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  25. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  26. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  27. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  28. Nolin JD, et al. 2017. JCI Insight. 2:e94929. PubMed
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  45. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  46. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  47. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  48. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
  49. Field CS, et al. 2018. Oncoimmunology. 7:e1376154. PubMed
  50. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  51. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  52. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  53. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  54. Hitchcock JR, et al. 2020. FEBS J. 287:250. PubMed
  55. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  56. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  57. Nascimento DC, et al. 2017. Nat Commun. 8:14919. PubMed
  58. Hoffmann FM, et al. 2018. Front Immunol. 9:1046. PubMed
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  65. Sun L, et al. 2020. Nat Commun. 2.229166667. PubMed
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  68. Matsuo K, et al. 2018. J Invest Dermatol. 138:1764. PubMed
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  70. Wikstrom M, et al. 2006. J Immunol. 177:913. PubMed
  71. Benson M, et al. 2007. J Exp Med. 204:1765. PubMed
  72. Pericolini E, et al. 2008. J Leukoc Biol. 83:1286. PubMed
  73. Osterholzer J, et al. 2009. J Immunol. 183:8044. PubMed
  74. Fahlén-Yrlid L, et al. 2009. J Immunol. 183:5032. PubMed
  75. Eisenach P, et al. 2010. J Cell Sci. 123:4182. PubMed
  76. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  77. Leppin K, et al. 2014. Invest Ophthalmol Vis Sci . 55:3603. PubMed
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RRID
AB_313770 (BioLegend Cat. No. 117301)
AB_313771 (BioLegend Cat. No. 117302)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD11c Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD11c N418 FC
Biotin anti-mouse CD11c N418 FC, IHC-F
FITC anti-mouse CD11c N418 FC
PE anti-mouse CD11c N418 FC
Purified anti-mouse CD11c N418 FC, CyTOF®, IP, IHC
Alexa Fluor® 488 anti-mouse CD11c N418 FC
Alexa Fluor® 647 anti-mouse CD11c N418 FC, IHC, ICC, 3D IHC
PE/Cyanine5 anti-mouse CD11c N418 FC
PE/Cyanine7 anti-mouse CD11c N418 FC
Brilliant Violet 605™ anti-mouse CD11c N418 FC
Alexa Fluor® 700 anti-mouse CD11c N418 FC
Pacific Blue™ anti-mouse CD11c N418 FC
APC/Cyanine7 anti-mouse CD11c N418 FC
PerCP/Cyanine5.5 anti-mouse CD11c N418 FC
PerCP anti-mouse CD11c N418 FC
Brilliant Violet 421™ anti-mouse CD11c N418 FC, IHC
Brilliant Violet 570™ anti-mouse CD11c N418 FC
Brilliant Violet 785™ anti-mouse CD11c N418 FC
Brilliant Violet 510™ anti-mouse CD11c N418 FC
Brilliant Violet 650™ anti-mouse CD11c N418 FC
Purified anti-mouse CD11c (Maxpar® Ready) N418 FC, CyTOF®
Alexa Fluor® 594 anti-mouse CD11c N418 IHC-F, 3D IHC
PE/Dazzle™ 594 anti-mouse CD11c N418 FC
Brilliant Violet 711™ anti-mouse CD11c N418 FC
APC/Fire™ 750 anti-mouse CD11c N418 FC
TotalSeq™-A0106 anti-mouse CD11c N418 PG
Brilliant Violet 750™ anti-mouse CD11c N418 FC
TotalSeq™-B0106 anti-mouse CD11c N418 PG
TotalSeq™-C0106 anti-mouse CD11c N418 PG
KIRAVIA Blue 520™ anti-mouse CD11c N418 FC
Spark Blue™ 550 anti-mouse CD11c N418 FC
Spark NIR™ 685 anti-mouse CD11c N418 FC
Spark UV™ 387 anti-mouse CD11c N418 FC
Spark Red™ 718 anti-mouse CD11c N418 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse CD11c

  • Biotin anti-mouse CD11c

  • FITC anti-mouse CD11c

  • PE anti-mouse CD11c

  • Purified anti-mouse CD11c

  • Alexa Fluor® 488 anti-mouse CD11c

  • Alexa Fluor® 647 anti-mouse CD11c

  • PE/Cyanine5 anti-mouse CD11c

  • PE/Cyanine7 anti-mouse CD11c

  • Brilliant Violet 605™ anti-mouse CD11c

  • Alexa Fluor® 700 anti-mouse CD11c

  • Pacific Blue™ anti-mouse CD11c

  • APC/Cyanine7 anti-mouse CD11c

  • PerCP/Cyanine5.5 anti-mouse CD11c

  • PerCP anti-mouse CD11c

  • Brilliant Violet 421™ anti-mouse CD11c

  • Brilliant Violet 570™ anti-mouse CD11c

  • Brilliant Violet 785™ anti-mouse CD11c

  • Brilliant Violet 510™ anti-mouse CD11c

  • Brilliant Violet 650™ anti-mouse CD11c

  • Purified anti-mouse CD11c (Maxpar® Ready)

  • Alexa Fluor® 594 anti-mouse CD11c

  • PE/Dazzle™ 594 anti-mouse CD11c

  • Brilliant Violet 711™ anti-mouse CD11c

  • APC/Fire™ 750 anti-mouse CD11c

  • TotalSeq™-A0106 anti-mouse CD11c

  • Brilliant Violet 750™ anti-mouse CD11c

  • TotalSeq™-B0106 anti-mouse CD11c

  • TotalSeq™-C0106 anti-mouse CD11c

  • KIRAVIA Blue 520™ anti-mouse CD11c

  • Spark Blue™ 550 anti-mouse CD11c

  • Spark NIR™ 685 anti-mouse CD11c

  • Spark UV™ 387 anti-mouse CD11c

  • Spark Red™ 718 anti-mouse CD11c
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