- TS2/16 (See other available formats)
- V A-S202
- Other Names
- Integrin β1 chain, VLA-β chain, gpIIa, ITGB1
- Mouse IgG1, κ
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- Product Citations
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CD29 is a 130 kD single chain type I glycoprotein also known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.Product Details
- Human, African Green, Baboon, Cattle (Bovine, Cow), Cynomolgus, Dog (Canine), (Horse reactivity), Rhesus
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Validated
- Recommended Usage
- This product is suitable for use with the Maxpar® Metal Labeling Kits. The product is formulated so that the buffer exchange step is not required (steps 7, 8, 9, and 10 in the Maxpar® antibody labeling protocol). Just add 100 µl of this antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter, as described in step 11, and continue with the protocol.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen tissue sections3,5, and activation of integrin β14,7,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.10
- Additional Product Notes
- Maxpar® is a registered trademark of Fluidigm Inc.
(PubMed link indicates BioLegend citation)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
- Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
- Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
- Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
- Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
- Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
- Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
- Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
- Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
- Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
- Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)
AB_2563738 (BioLegend Cat. No. 303021)
- Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α1-α6), also associates with CD51 (αV), and α7- α9, 130 kD
Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells
- Cell-cell and cell-matrix interactions
- VCAM-1, MAdCAM-1, ECM
- Cell Type
- Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Monocytes, Platelets, Mesenchymal Stem Cells, Embryonic Stem Cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Hemler M. 1990. Annu. Rev. Immunol. 8:365.
2. Hynes R. 1992. Cell 69:11.
- Gene ID
- 3688 View all products for this Gene ID
- View information about CD29 on UniProt.org
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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