Purified anti-human ACE2 Antibody

Pricing & Availability
Clone
A20069I (See other available formats)
Other Names
Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACE-2, ACEH
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A20069I_PURE_ACE2_Antibody_1_061720.png
Human paraffin-embedded kidney tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human ACE2 (clone A20069I), and Nestin (clone 10C2) Alexa Fluor® 594 (green) at 4°C overnight, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • A20069I_PURE_ACE2_Antibody_1_061720.png
    Human paraffin-embedded kidney tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human ACE2 (clone A20069I), and Nestin (clone 10C2) Alexa Fluor® 594 (green) at 4°C overnight, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • A20069I_PURE_ACE2_Antibody_2_061720.png
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human ACE2 (clone A20069I), and anti-human CD45RA (clone HI100) Alexa Fluor® 594 (green) at 4°C overnight, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • A20069I_PURE_ACE2_Antibody_3_061720.png
    Human paraffin-embedded testis tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human ACE2 (clone A20069I) at 4°C overnight, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • A20069I_PURE_ACE2_Antibody_4_061720.png
    Whole cell extracts (15 µg protein) from HeLa cells (negative control), human kidney and human testis tissue (positive controls), and mouse kidney tissue were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. Membranes were probed with 0.1 µg/mL (1:5000 dilution) of purified anti-human ACE2 antibody (clone A20069I) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
Cat # Size Price Quantity Avail. Save
375801 25 µg $105
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375802 100 µg $265
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Description

Angiotensin-converting enzyme 2 (ACE2) is a type one transmembrane protein, which shares approximately 60% homology with ACE. The predicted human ACE2 protein sequence consists of 805 amino acids, including an N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE2 catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7 involved in vasoconstriction. Recently, ACE2 has been shown to be a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus). Based on recent single-cell RNA sequencing (scRNA-Seq) database, high ACE2 RNA expression was identified in type II alveolar cells (AT2) of lung, esophagus upper and stratified epithelial cells, kidney proximal tubule cells, enterocytes in ileum and colon, cholangiocyte in bile duct, myocardial cells in heart, and endothelium and vascular smooth muscle cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein cover extracellular domain of ACE2 (Gln 18-Ser740)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2860958 (BioLegend Cat. No. 375801)
AB_2860959 (BioLegend Cat. No. 375802)

Antigen Details

Structure
Type one transmemberance protein. Protein sequence consistent extracellular domain (Gln 18-Ser740 ) contain cytalitic domain, transmembrane domain (AA741-AA761) and cytoplasmic domain (AA762-AA805).
Distribution

Type II alveolar cells (AT2) of lung, esophagus upper and stratified epithelial cells, kidney proximal tubule cells,  enterocytes in ileum and colon, cholangiocyte in bile duct, myocardial cells in heart, endothelium and vascular smooth muscle cells

Function
Catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7 involved in vasoconstriction
Interaction
Angiotensin II, ADAM17, TMPRSS2
Ligand/Receptor
Functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus)
Cell Type
Monocytes
Biology Area
Angiogenesis, COVID-19, Immunology
Molecular Family
Enzymes and Regulators
Antigen References
  1. South AM, et al. 2020. Nat Rev Nephrol. 16:305-7.
  2. Hoffmann M, et al. 2020. Cell. 181:271-80.
  3. Sungnak W, et al. 2020. Nat Med. 26:681-7.
  4. Li W, et al. 2003. Nature. 426:450-4.
  5. Heurich A, et al. 2014. J Virol. 88:1293-30.
  6. Hamming I, et al. 2004. J Pathol. 203:631-7.
Gene ID
59272 View all products for this Gene ID
UniProt
View information about ACE2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/16/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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