Ultra-LEAF™ Purified anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
H22 (See other available formats)
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
H22_021711
Recombinant Mouse IFN-γ was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-mouse IFN-γ antibody (clone H22). Proteins were visualized using a goat anti-Armenian hamster IgG secondary conjugated to HRP and chemiluminescence detection.
  • H22_021711
    Recombinant Mouse IFN-γ was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-mouse IFN-γ antibody (clone H22). Proteins were visualized using a goat anti-Armenian hamster IgG secondary conjugated to HRP and chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
513207 100 µg $100
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513208 1 mg $135
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513209 5 mg $490
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513210 25 mg $1,280
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513211 50 mg $2,140
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513212 100 mg $3,075
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Recombinant full-length murine IFN-γ
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

WB - Quality tested
Neut, ELISA, IF, IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified H22 antibody is useful in mouse IFN-γ ELISA, immunofluorescence and immunoprecipitation applications. For in vitro or in vivo neutralization applications, it is suggested to use LEAF™ Purified H22. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Schreiber RD, et al. 1985. J. Immunol. 134:1609.
  2. Williams RO, et al. 1993. Clin. Exp. Immunol. 92:323
  3. Steed AL, et al. 2006. J. Virol. 80:192.
  4. Wu LL, et al. 2014. Am J Pathol. 184:2260. PubMed

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Function
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Interaction
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571
4. Gray P, et al. 1987. Lymphokines 13:151

Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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