Purified anti-CD34 Antibody (Previously Covance catalog# SIG-3326)

Pricing & Availability
Clone
QBEnd/10 (See other available formats)
Regulatory Status
RUO
Other Names
Hematopoietic progenitor cell antigen CD34, CD34 antigen
Previously
Signet Catalog# 326-26 L2 Predilute
Covance Catalog# SIG-3326
Isotype
Mouse IgG1, κ
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Product Citations
publications
QBEndslash10_Purified_CD34_Antibody_IHC_021715
Robust cytoplasmic and membrane staining of endothelial cells in frozen human placenta with antibody CD34.
  • QBEndslash10_Purified_CD34_Antibody_IHC_021715
    Robust cytoplasmic and membrane staining of endothelial cells in frozen human placenta with antibody CD34.
Cat # Size Price Quantity Avail. Save
826401 50 µL $179.00
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

 

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated using a vesicular suspension of a perfusate of human term placenta.
Formulation
Phosphate-buffered solution with BSA + 0.1% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

IHC

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:1000-1:1500 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Formalin-fixed, paraffin-embedded tissues, frozen sections
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 1 (Cat. No. 927901).
Incubation: 20 minutes at room temperature

Application Notes

This antibody is effective in immunohistochemistry (IHC).

The BioLegend QBEnd/10 monoclonal antibody detects the CD34 antigen. It stains the cytoplasm of endothelial cells of vascular tissue with some cross reactivity to basement membrane collagen. In tumors this monoclonal antibody is valuable in labelling the vascular endothelium of Kaposi's sarcoma, tumors involving the liver, spindle shaped tumors and any other highly vascular neoplastic or normal tissue.

**We recommend a quick spin prior to opening the vial.
Positive tissue (human): Skin, tonsil or other highly vascular tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Anthony P, et al. Endothelial markers in malignant vascular tumor of the liver: superiority of QBEnd/10 over von willebrand factor and uelex europus agglutinin 1. J Clin Path 44:2, 1991.
  2. Sankey EA, et al. QBEnd/10: a new immunostain for the diagnosis of Kaposi's Sarcoma. J Path 161:267-271, 1990.
  3. Fletcher CDM, et al. QBEnd/10: a useful but by no means a specific marker for the diagnosis of kaposi's sarcoma. J Path 162:274, 1990.
  4. Ramani P, et al. QBEnd/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections. Histopathol 17:237-242, 1990.
RRID
AB_2564903 (BioLegend Cat. No. 826401)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selectin, E-selectin
Cell Type
Hematopoietic stem and progenitors, Endothelial cells, Fibroblasts
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Antigen References

1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.

Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03/15/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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