- P5D2 (See other available formats)
- Other Names
- FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB, glycoprotein IIa, integrin VLA-4 beta subunit, integrin beta-1, very late activation protein, beta polypeptide
- Mouse IgG1, κ
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- Product Citations
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CD29 is a 130 kD single chain type I glycoprotein also known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.Product Details
- Antibody Type
- Host Species
- The P5D2 monoclonal antibody was generated against human fibroblast cells (HT1080).
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
Block - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone P5D2 interferes with binding of integrin-bearing cells to fibronectin, laminin and collagen but not vitronectin coated surfaces.
P5D2 reacts with the integrin β1 subunit and inhibits the function of all β1 integrins.
(PubMed link indicates BioLegend citation)
- Wayner EA, Hoffstrom BG. 2007. Meths Enzymol In: Integrins. 426:117. PubMed
- Wang HQ, et al. 2007. Eur J Cell Biol. 86(1):51. (Block) PubMed
- Da Silva MS, et al. 2003. Surgery. 134(2):164. (Block) PubMed
- Blaschke R, et al. 2002. Biochem Biophys Res Commun. 296(4):890. (Block) PubMed
- Dittel BN, et al. 1993. Blood. 81:2272. (FC) PubMed
- Product Citations
AB_2650837 (BioLegend Cat. No. 921303)
AB_2650620 (BioLegend Cat. No. 921304)
- CD29 is a 130 kD single chain type I glycoprotein.
- Tissue distribution: Leukocytes, endothelial cells, smooth muscle, epithelial cells, central nervous system, and stem cells.Cellular distribution: endosome, extracellular, plasma membrane, and cytoskeleton.
- Cell-cell and cell-matrix interactions
- VCAM-1, MAdCAM-1, extracellular matrix (ECM)
- Cell Type
- Leukocytes, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, Embryonic Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Signal Transduction, Stem Cells, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Long K, et al. 2016. Nat. Commun. 7:10354. PubMed
2. Campos LS. 2005. Bioessays. 27(7):698.
- Gene ID
- 3688 View all products for this Gene ID
- View information about CD29 on UniProt.org
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