PerCP/Cyanine5.5 anti-mouse CD49a Antibody

Pricing & Availability
Clone
HMα1 (See other available formats)
Other Names
Very late antigen 1 (VLA1), integrin alpha 1 (ITGA1)
Isotype
Armenian Hamster IgG
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Product Citations
publications
HMa1_PerCPCyanine55_CD49a_Antibody_081618
C1300 (mouse neuroblastoma cell line) cells were stained with CD49a (clone HMα1) PerCP/Cyanine5.5 (filled histogram) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
  • HMa1_PerCPCyanine55_CD49a_Antibody_081618
    C1300 (mouse neuroblastoma cell line) cells were stained with CD49a (clone HMα1) PerCP/Cyanine5.5 (filled histogram) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
142611 25 µg $110
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142612 100 µg $260
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Description

CD49a is a 1179 aa, type I transmembrane glycoprotein also known as α1 integrin, VLA-1 α chain, or integrin α1. It associates antibody v042010 with CD29 (β1 integrin) to form the VLA-1 complex, a collagen IV and alminin-1 receptor that is expressed on activated T cells, smooth muscle cells, endothelial cells, neuronal cells, fibroblasts, and mesenchymal cells. CD49a is an adhesion molecule and is involved in the regulation of leukocyte migration, T cell proliferation, and cytokine production.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse Neuroblastoma Cell Line C1300
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant format) include: inhibition of cell adhesion and cytokine production1,2.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (FC, Block)
  2. Tanaka T, et al. 1995. Int. Immunol. 7:1183. (Block)
RRID
AB_2750218 (BioLegend Cat. No. 142611)
AB_2750219 (BioLegend Cat. No. 142612)

Antigen Details

Structure
1179 aa, type I transmembrane glycoprotein
Distribution

Endothelial cells, fibroblasts, activated T cells

Function
Leukocyte adhesion and homing; costimulation
Ligand/Receptor
Collagens, laminin
Cell Type
Endothelial cells, Fibroblasts, T cells, Mesenchymal Stem Cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Signal Transduction, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barczyk M, et al. 2010. Cell Tissue Res. 339:269.

Gene ID
109700 View all products for this Gene ID
UniProt
View information about CD49a on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 08/16/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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