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- Clone
- S20020G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- S152, T14, TNFRSF7
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes stained with anti-human CD3 FITC and anti-human CD27 (clone S20020G) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 384903 | 25 tests | $150 | ||||
| 384904 | 100 tests | $330 | ||||
CD27, also known as S152 and T14, is a 50-55 kD type I membrane protein. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Hu CD27 transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone S20020G is able to completely block the binding of clones M-T271 and O323 on target cells. Clone S20020G does not block binding of clone S20020H on target cells.
- RRID
-
AB_2936745 (BioLegend Cat. No. 384903)
AB_2936745 (BioLegend Cat. No. 384904)
Antigen Details
- Structure
- TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
- Distribution
-
Medullary thymocytes, T and B cell subsets, NK cells
- Function
- T cell costimulation
- Interaction
- TRAF2, TRAF5
- Ligand/Receptor
- CD70
- Cell Type
- B cells, Lymphocytes, NK cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Camerini D, et al. 1991. J Immunol. 147:3156
- Hintzen R, et al. 1994. Immunol Today. 15:307-11.
- Gene ID
- 939 View all products for this Gene ID
- UniProt
- View information about CD27 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
Other Formats
View All CD27 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE/Cyanine7 anti-human CD27 | S20020G | FC |
| Purified anti-human CD27 | S20020G | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE/Cyanine7 anti-human CD27
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Purified anti-human CD27
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