PE anti-Syk Antibody

Pricing & Availability
Clone
5F5 (See other available formats)
Other Names
Spleen tyrosine kinase, protein tyrosine kinase SYK
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
5F5_PE_Syk_ICFC_1_021315
C57BL/6 mouse splenocytes were surface stained with CD19 Brilliant Violet 421™, then treated with the Nuclear Factor Fixation and Permeabilization Buffer Set, and intracellularly stained with anti-Syk (clone 5F5) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 5F5_PE_Syk_ICFC_1_021315
    C57BL/6 mouse splenocytes were surface stained with CD19 Brilliant Violet 421™, then treated with the Nuclear Factor Fixation and Permeabilization Buffer Set, and intracellularly stained with anti-Syk (clone 5F5) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 5F5_PE_Syk_ICFC_2_021315
See PE spectral data
Cat # Size Price Quantity Avail. Save
646003 25 µg $125
Check Availability


Need larger quantities of this item?
Request Bulk Quote
646004 100 µg $295
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Syk peptide aa306-333
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.03 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Chu D, et al. 1999. J. Immunol. 163:2610.
  2. Ackermann JA, et al. 2015. J Immunol. 194:4650. PubMed
Product Citations
  1. Luo W et al. 2018. Immunity. 48(2):313-326 . PubMed
  2. Li X, et al. 2018. Cell Host Microbe. 24:847. PubMed
RRID
AB_2565305 (BioLegend Cat. No. 646003)
AB_2565306 (BioLegend Cat. No. 646004)

Antigen Details

Structure
Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
Distribution

Cytoplasmic protein, widely expressed in hematopoietic cells

Function
Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790.
2. Toyabe SL, et al. 2001.Immunology 103:164.

Gene ID
6850 View all products for this Gene ID
UniProt
View information about Syk on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02/13/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Forgot your password? Reset Password
Request an Account