- 5F5 (See other available formats)
- Regulatory Status
- Other Names
- Spleen tyrosine kinase, protein tyrosine kinase SYK
- Mouse IgG1, κ
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- Product Citations
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Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse Syk peptide aa306-333
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
- Additional Product Notes
In the first WB figure above, the cell lines selected for antibody testing were chosen to demonstrate specificity and species cross-reactivity, A549 was chosen as a human control cell line that does not express Syk protein; RPMI 8225 and Daudi are human cell lines that express Syk protein; and A20 cells is a mouse cell line that expresses Syk protein. Predicted SYK expression was obtained from Human Protein Atlas.
(PubMed link indicates BioLegend citation)
- Chu D, et al. 1999. J. Immunol. 163:2610.
- Ackermann JA, et al. 2015. J Immunol. 194:4650. PubMed
- Product Citations
AB_1659262 (BioLegend Cat. No. 646001)
AB_1659261 (BioLegend Cat. No. 646002)
- Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
Cytoplasmic protein, widely expressed in hematopoietic cells
- Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
- Biology Area
- Cell Biology, Signal Transduction
- Antigen References
1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790.
2. Toyabe SL, et al. 2001.Immunology 103:164.
- Gene ID
- 6850 View all products for this Gene ID
- View information about Syk on UniProt.org
Related Pages & Pathways
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